ECE2018 Poster Presentations: Diabetes, Obesity and Metabolism Obesity (78 abstracts)
Activation of p53 with low doses doxorubicin reduce the acumulation of lipids in two in vitro models of liver steatosis
Marcos Fernandez Fondevila 1 , Begoña Porteiro Coto 1 , Xabier Buque 2 , María Jesús González Rellán 1 , Uxía Fernández Paz 1 , Alfonso Mora 3 , Daniel Beiroa 1 , Ana Senra 1 , Rosalía Gallego 4 , Miguel López 1 , Guadalupe Sabio 3 , Carlos Diéguez 1 , Patricia Aspichueta 2 & Rubén Nogueiras Pozo 1
1Department of Physiology, CIMUS, University of Santiago de Compostela, Santiago de Compostela, Spain; 2Biocruces Research Institute, Bizcaia, Spain; 3Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain; 4Department of Morphological Sciences of Santiago de Compostela, Santiago de Compostela, Spain.
Introduction: p53 is transcription factor widely known because of its antitumoral actions. New evidences suggest that p53 also play a key role in the regulation of metabolic homeostasis and specifically in the lipid metabolism.
Objective: We hypothesize that the chemical activation of p53 with low doses of doxorubicin could ameliorate the lipid metabolism in in vitro models of liver steatosis.
Methods: We treated with low concentrations of doxorubicin two human hepatic cell lines, HepG2 and THLE-2, exposed to oleic acid to induce lipid accumulation. Furthermore, we administered doxorubicin to HepG2 cells downregulating p53 with siRNAs.
Results: The doxorubicin treatment reduced the lipid accumulation in two human hepatic cell lines in a p53-dependent manner. The drug stimulated the lipid oxidation and inhibited the de novo lipogenesis at concentrations that did not affect the cell viability or apoptosis.
Conclusion: The activation of p53 with low doses of doxorubicin could provide a new strategy to reduce the lipid accumulation in the liver of patients with hepatic steatosis.
Acknowledgements: This work has been supported by the predoctoral fellowship from Ministerio de Educación, Cultura y Deporte, through the Plan Estatal de Investigación Científica y Técnica y de Innovación 2013–2016.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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