Endocrine Abstracts

Objective: Dietary total antioxidant capacity (TAC) has been assumed as a useful tool to consider the relationship between the cumulative antioxidant food capacity and several chronic disorders. The contribution of p53 to many aspects of age-associated diseases, such as cardiovascular and metabolic disorders has been recognized. The role of dietary TAC in adipose tissues metabolism especially its association with P53 gene expression is not established yet in fat mass. The aim of the study was to investigate the association of dietary TAC intake with p53 gene expression in omental and subcutaneous adipose tissues of obese and non-obese adults.

Methods: In this cross-sectional study, omental and subcutaneous adipose tissues of 98 non-diabetic participants including 44 subjects with BMI <30 and 54 subjects with BMI ≥30 kg/m² were collected from who had undergone elective abdominal surgery. Usual intake of participants was assessed by a relative and validated semi-quantitative food-frequency questionnaire (FFQ) before the surgery. Dietary TAC intake was assessed based on the oxygen radical absorbance capacity method. The p53 gene expression was measured by using SYBR Green Real-Time PCR.

Results: The mean age of non-obese and obese participants was 46.3±15.3 and 37.9±11.6 years, respectively, which was significantly different (P=0.003). The dietary TAC intake was 317.6 and 288.7 μmolTE/100g in obese and non-obese participants, respectively (P=0.434). The mRNA levels of p53 in subcutaneous adipose tissues was 0.77±4.84 and −0.06±4.61 in obese and non-obese participants, respectively (P=0.397) and in omental adipose tissue was −0.23±4.50 and 1.35±4.00 in obese and non-obese ones, respectively (P=0.078). After controlling for age and total energy intake, TAC positively associated with subcutaneous adipose tissue p53 mRNA levels in obese participants (B=0.274, P=0.043). There was no significant association in omental adipose tissues (B=0.005, P=0.974).

Conclusion: Higher intake of TAC in the regular diet was associated with p53 gene expression in the subcutaneous adipose tissue; supporting this idea, that overall antioxidant capacity of diet is by affecting the intracellular concentration of p53 protects one from the development of carcinoma.