ECE2018 Poster Presentations: Diabetes, Obesity and Metabolism Diabetes (to include epidemiology, pathophysiology) (73 abstracts)
1Internal Medicine department, Cairo University, Cairo, Egypt; 2Clinical Pathology, Cairo University, Cairo, Egypt.
Background: Studies from different parts of the world have given controversial results regarding the association of transcription factor 7like 2 (TCF7L2) gene polymorphism with Type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN). Aim: This study aimed to investigate if TCF7L2 gene polymorphism, rs12255372(G>T) is a risk factor for the development of T2DM and DN in type 2 Egyptian diabetic patients. PATIENTS AND METHODS: This study was conducted on 150 subjects, divided into three groups (50 type-2 diabetic patients without nephropathy, 50 type-2 diabetic patients with nephropathy (DN) and 50 age and sex matched normal subjects). Genotyping for the rs 12255372(G>T) polymorphism in TCF7L2 gene was performed by real time PCR. RESULTS: There was statistically significant association between TCF7L2 gene polymorphism rs 12255372 and type 2 diabetic patients & DN, P=0.005, the allelic frequency differed significantly between the three studied groups P=0.005, denoting that the G allele was the risky allele for developing T2DM & DN. CONCLUSION: The present study indicates a strong association between TCF7L2 variants and the risk of developing T2DM & DN and suggests a role of ethnicity and genetic background for susceptibility to diabetes and its complications.
Keywords: Type-2 diabetes mellitus diabetic nephropathy TCF7L2 Gene single nucleotide polymorphism real time PCR.