Endocrine Abstracts

Miscarriage is a very common occurrence in humans, in which blastocyst implantation failure in naturally-occurring and assisted human reproduction occurs in up to 2/3 of all cases. Calcium (Ca²⁺) has been showed to involve in many cellular signal transduction pathways as well as regulation of cell adhesion, which is necessary for the physiology process of endometrial epithelial cell transformation and stromal cell decidualization during embryo implantation. Previous studies have been reported that EDC can regulate the expression of genes associated with calcium transport in during pregnancy period such as TRPV6, PMCA, TRPV5, NCX1 and CaBP-D9k. Additionally, exposure to EDs during early gestation results in disrupt intrauterine implantation, uterine receptive, leading to implantation failure. In this study, BPA, OP, E2 and/or ICI 172,80 (antagonist) were inject by subcutaneous from 1 to 3 day post coitus. The number of implantation sites were significant decreased in OP group and no implantation site was observed in EDC+ICI groups. There was different in the expression of Ca channel between maternal uterine and implantation. The level of TRPV6, TRPV5 mRNA in uterus were significantly increased by EDC and/or ICI treatment. In contrary, their expressions are significantly decreased by OP- and BPA- treated in implantation site. The expression of NCX1 and PMCA1 mRNA level were significantly decreased in OP and BPA groups than that in the VE group. The expression of Muc-1 mRNA, a major epithelial apical surface glycoprotein expressed significantly decreased by OP-treated in implantation site. The mRNA level of E-cadherin, a group of cell surface glycoproteins responsible for maintaining adhesion between epithelial cells using a calcium-dependent mechanism was also significantly decreased in implantation site by BPA and OP treatment. Taken together, BPA and OP regulate the expression of calcium channel during early pregnancy period, throughout induce the loss numbers of implantation via reduce the uterine conceptive and implantation adhesion and invasion.