Endocrine Abstracts

Laser Doppler fluorometry (LDF) is not an invasive method for diagnosing microcirculation in patients with diabetes mellitus, but the method needs to be improved. Evaluation of the LDF method in patients with diabetic neuropathy (DN) before and after the appointment of vitamin D (VD). Included patients with DM and VD deficiency, DM duration more then 5 years, HbA1c up to 9.0%, non-smokers, signed IC. LAKK-M was used. The value of perfusion was measured-M, σ- average modulation of blood flow and CV –coefficient of variation, two functional tests (postural and occlusive). Patients without changing hypoglycemic treatments were randomized into three groups: 5000 IU (group I) and 40 000 IU (group II) per week cholecalciferol treatment and control group (group III). The duration of observation is 24 weeks. Group I included 12 patients (6F) at the age of 56.1 (±5.4) with an average HbA1c of 8.17±0.36% and vitamin 25 (OH) D of 21.4±3.4 nmol/l. Group II included 12 patients (6F), the mean age was 51.3 (±5.9), HbA1c 8.35±0.63%, 25 (OH) D 20.9±4.1 nmol/l. Group III included 14 patients (7F) HbA1c 7.32±0.19% and 25 (OH) D 27.1±1.9 nmol/l. The initial parameters of microcirculation did not differ in the three groups (data given in I-II- III groups): M=10.63±1.37, 12.36 (±2.51), 12.36 (±1.92), σ=5.32±0.79, 4.44±1, 05, 7.1±0.38, Kv =11.36±1.59, 10.64±2.93, 15.44±2.49. After 24 weeks HD we found no changes in group I-HbA1c 8.06±0.39%, 25 (OH) D 24.68 (P=0.07), M=10.30±1.14 (P=0.08), σ=4, 98±0, 56 (P=0.05) and Kv =11.82±2.38 (P=0.87). Significant differences were found in group II: HbA1c decreased to 7.42±0.73% (P=0.023), 25 (OH) D increased to 60.88 (P=0.003); All parameters were improved microcirculation M=19.69±2.52 (P=0.003), σ=6.05±0.93 (P=0.005), Kv =13.36±3.15 (P=0.017). The indices of group III did not change statistically significant (P=0.46). The LDF method for the diagnosis of changes in microcirculation DN was informative in conjunction with functional tests. diabetes mellitus; laser dopplerography; Vitamin D