ECE2018 Poster Presentations: Calcium and Bone Calcium & Vitamin D metabolism (59 abstracts)
Quantification of serum 25-hydroxyvitamin D: a comparison between competitive chemiluminescence immunoassay and mass spectrometry coupled to high performances liquid chromatography
Federica Saponaro 1 , Sabina Frascarelli 1 , Alessandro Saba 1 , Concetta Prontera 2 , Aldo Clerico 2 , Marco Scalese 3 , Claudio Passino 2 , Claudio Marcocci 4 & Riccardo Zucchi 1
1Laboratory of Biochemistry, Department of Surgical, Medical, Molecular and Critical Area Pathology, University of Pisa, Pisa, Italy; 2Fondazione Toscana Gabriele Monasterio, Pisa, Italy; 3Institute of Clinical Physiology, National Council of Research, Pisa, Italy; 4Endocrinology Unit 2, University Hospital of Pisa, Pisa, Italy.
Serum 25-hydroxy-vitamin D (25OHD) is considered the most reliable marker of vitamin D status. Adequate levels 25OHD of are necessary for pleiotropic effects of vitamin D, either skeletal or extra-skeletal. Traditional assays based on immunoassay often show an unsatisfactory accuracy and sensibility. A valuable alternative is Tandem Mass Spectrometry coupled to High Performances Liquid Chromatography (HPLC-MS-MS), that offers a good quantification accuracy, as the contribution of interfering compounds to the final results is limited. We enrolled 110 consecutive patients with Heart Failure, who underwent comprehensive biohumoral characterization. The analyses of 25OHD by chemiluminescence immunoassay (DiaSorin LIAISON) and HPLC-MS-MS were performed at the same moment from two aliquots of the same stored sample. 25OHD levels with LIAISON were statistically lower than with HPLC-MS-MS (17.6±8.9 ng/ml vs 18.9±9.4, P<0.0001). The prevalence of Vitamin D insufficiency (<50 nmol/l or 20 ng/ml) was statistically lower using HPLC-MS-MS compared to LIAISON (59% vs 63%, P<0.0001). The same result was found for severe Vitamin D deficiency (<25 nmol/l or 10 ng/ml; 20.9% vs 25.4%=<0.001). A good correlation (R=0.909, R2=0.824, P<0.001) between 25OHD values measured with LIAISON and with HPLC-MS-MS was found. The inter-assay bias was evaluated by Bland-Altman plots: compared to the HPLC-MS-MS method, LIAISON assay demonstrated a mean relative bias of −6.54% with 95% of limits of agreement (−46.52% to +33.44%). HPLC-MS-MS technology is well correlated to the method currently used (CLIA), avoid overestimation of hypovitaminosis D and is a reliable diagnostic tool for 25OHD measurement.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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