ECE2017 Eposter Presentations: Adrenal and Neuroendocrine Tumours Adrenal cortex (to include Cushing's) (86 abstracts)
Salivary steroid levels during diagnostic tests for adrenal insufficiency
Michaela Dušková 1 , Kateřina Šimůnková 1, , Jana Vítků 1 , Lucie Kolátorová 1 , Hana Jandíková 1 , Hana Pospíšilová 1 , Monika Šrámková 1 , Mikuláš Kosák 2 & Luboslav Stárka 1
1Institute of Endocrinology, Praha, Czech Republic; 2Third Department of Medicine, General University Hospital and First Faculty of Medicine, Charles University, Praha, Czech Republic.
Diagnostic tests of the hypothalamic–pituitary–adrenal axis (HPA axis), measuring of free cortisol and use of other adrenal steroids are the subject of debate.
We investigated 15 healthy men with mean/median age 27.4/26 (±4.8) years, and mean/median BMI 25.38/24.82 (±3.2) kg/m2. All subjects underwent four dynamic tests of the HPA axis, specifically 1, 10, and 250 μg synacthen tests and an insulin tolerance test (ITT). Salivary cortisol, cortisone, pregnenolone, and DHEA were analysed using liquid chromatography-tandem mass spectrometry.
During the ITT maximum salivary cortisol levels of over 12.5 nmol/l were found at 60 min. Maximum cortisol levels in all of the Synacthen tests were higher than stimulation in the ITT. Cortisone reacted similarly as cortisol, i.e. we did not find any change in the ratio of cortisol to cortisone. Pregnenolone and DHEA were higher during the ITT, and their peaks preceded the cortisol peak. There was no increase of pregnenolone or DHEA in any of the Synacthen tests.
Our results indicate that a 10 μg Synacthen dose is a safe and cost-effective choice.
This study was supported by the project MZCR for conceptual development of research organization 00023761 Institute of Endocrinology and grant 17-28692A.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more