ECE2017 Oral Communications Receptors & Signalling (5 abstracts)
Epigenetic regulation of aldosterone synthase gene, CYP11B2 by potassium
Yoshiyu Takeda , Masashi Demura , Takashi Yoneda , Yoshimichi Takeda , Mitsuhiro Kometani , Seigo Konishi & Toshitaka Sawamura
Kanazawa University, Kanazawa, Japan.
Purpose: We found the hypomethylation status of CYP11B2 gene in the aldosterone-producing adenomas compared with normal adrenal glands or non-functioning adrenal adenomas and reported that the methylation of promoter region of this gene influenced the mRNA expression in the adrenal cells. Angiotensin II or potassium regulates CYP11B2 gene expression in the adrenal cells. In order to clarify the acute effect of potassium on the methylation status of CYP11B2 gene, Human adrenal cells rats were treated with potassium and the methylation status of CYP11B2 gene in the adrenal cells was examined.
Methods: H295R cells were treated with potassium for 28 days. The gene expression of CYP11B2 was measured by real time quantitative PCR in the adrenal cells. ChiP assay and methylation activity were measured. Isolated DNAs from adrenal cells were treated with bisulfite and amplified using primers specific for the human CYP11B2 promoter regions.
Results: CYP11B2 mRNA levels were increased from 4th day to 7th day by the treatment with potassium. The methylation ratio was decreased from 4th day. The expression of CREB1 and NR4A1 were increased from 4th day. The methylation activity was increased from 7th day. The chromatin accessibility was increased from 4th day to 7th day
Conclusions: Aldosterone biosynthesis may be reversibly regulated by the epigenetic mechanism.
Volume 49
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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