Endocrine Abstracts

Introduction: The beneficial interaction between the microbiota and humans is the way how bacteria contained within the gut ‘talk’ to the immune system. Into this landscape probiotics and nutraceuticals play a major role. The study aims to determine whether probiotics plus nutraceuticals such as smectite or omega-3 are superior to probiotic alone on the monosodium glutamate (MSG) induced obesity model in rats.

Methods: Totally 75 rats divided into five groups were included (n=15, in each). Rats of group I were intact. Newborns rats of groups II-V were injected with MSG. The III (Symbiter) group received 2.5 ml/kg of multiprobiotic “Symbiter” containing concentrated biomass of 14 probiotic bacteria genera. The IV (Symbiter-Omega) and V (Symbiter+Smectite) groups received combination of probiotic biomass supplemented with flax and wheat germ oil (250 mg of each, concentration of omega-3 fatty acids 1–5%) or smectite gel (250 mg) respectively. Anthropometric, biochemical parameters of lipid and carbohydrate metabolism, and level of proinflammatory cytokines (IL)-1β, IL-12Bp40, INF-γ) and anti-inflammatory cytokines (IL-4, IL-10, TGF-β) were measured.

Results: In all interventional groups significant reduction of total body and VAT weight as compared to MSG-obesity were observed. However, the lowest prevalence of obesity was noted for Symbiter-Omega (20% vs 33.3% as compared to other interventional groups). Moreover, supplementation of probiotics with omega-3 lead to more pronounced decreasing of HOMA-IR (2.31±0.13 vs 4.02±0.33, P<0.001) and elevation of adiponectin level (5.67±0.39 vs 2.61±0.27, P<0.001) as compared to obesity group. Both nutraceuticals combination with probiotic and probiotic alone equally attenuated inflammation that was confirmed by the decrease of the pro-inflammatory and the activation of anti-inflammatory system.

Conclusion: Probiotics and nutraceuticals led to a significantly lower prevalence of obesity, reduction of insulin resistance, total and VAT weight. Our study demonstrated that supplementation of probiotics with omega-3 may have most beneficial antiobesity properties.