ECE2017 Guided Posters Adrenal 3 (12 abstracts)
Increased exosomes in endogeneous Cushing syndrome
Gloria Aranda 1 , Gemma Xifra 2 , Jose Manuel Fernandez-Real 2 , Guillermo Garcia-Eguren 1 , Gregori Casals 3 , Adriana Pane 1 , Mireia Mora 1 , Irene Halperin 1 & Felicia A Hanzu 1
1Group of Endocrine Disorders, Department of Endocrinology Hospital Clinic Barcelona, IDIBAPS, CIBERDEM, Barcelona, Spain; 2Department of Endocrinology, Hospital de Girona ’Dr Josep Trueta’; IdIBGi Girona, CIBERobn, Girona, Spain; 3Biochemistry and Molecular Genetics, Hospital Clinic Barcelona, Barcelona, Spain.
Exosomes are nano-sized vesicles secreted by the cells in the exatracellular fluids representing a novel way of intracellular communication. Exosomes secreted from blood cells like monocytes and platetes have been reported to be checkpoints involved in haemostatic and thrombotic response. Glucocorticoids are inducing a profound alteration of blood cells homeostasis with atherothrombotic, bleeding and immune response alterations. Hereby we quantified for the first time circulating exosomes in overt Cushing syndrome (CS) patients.
Methods: Cross sectional study in CS patients and controls. Diagnosis of CS was performed following ECE and Endo guidelines. Blood samples from active, drug naive CS patients (n=20) and age and sex matched controls (n=20) were obtained after overnight fast. Peripheral blood cells, biochemical and hormonal parameters were analyzed using standard methods. Circulatory exosomes were quantified in plasma by a sandwich enzyme-linked-immuno-sorbent assay (ExoTEST) employing a monoclonal antihuman CD9 antibody. Further exosome phenotyping and analysis is ongoing.
Results: CS patients present as compared to controls a significant increased number of CD9 positive exosomes (2.35×1010 vs 7.74×109/100 μl; P=0.005**). Absolute monocytes (MN) number and 24 h urinary free cortisol (UFC) level correlates with the number of exosomes, respectively P=0.022* with r:0.391 for MN and P=0.005** with an r:0.467 for UFC.
Conclusion: Circulatory exosomes are increased in the hypercortisolemic state of Cushing syndrome.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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