ECE2017 Eposter Presentations: Thyroid Thyroid (non-cancer) (260 abstracts)
Measurement of anti-thyroglobulin antibodies, a matter of equipment?
Ricardo Capitão 1 , Susana Prazeres 2 , Ana Font 2 , Conceição Godinho 3 , Isaura Rodrigues 3 , Frederico Cerveira 4 , Graça Belo 1 , João Viana 1 & Deolinda Madureira 5
1Hospital Egas MonizCentro Hospitalar Lisboa Ocidental, Lisboa, Portugal; 2Instituto Português de Oncologia de Lisboa Francisco Gentil, Lisboa, Portugal; 3Centro Hospitalar Lisboa Central, Lisboa, Portugal; 4Centro Hospitalar do Baixo Vouga, Aveiro, Portugal; 5Sociedade Portuguesa de Endocrinologia Diabetes e Metabolismo, Lisboa, Portugal.
Introduction: The presence of anti-thyroglobulin antibodies (ATG) can induce falsely lower thyroglobulin (Tg) values. The methods characteristics used for ATG measurement vary according to the equipment/reagent used. ATG at our centre is measured by Unicap.
Objective: To assess if patients with negative ATG by Unicap® have different results with other equipment.
Methods: A cross-sectional retrospective study was conducted in February-March of 2016, in which blood samples from patients followed at our Centre, with negative ATG, were selected and separated into three groups: 1) differentiated thyroid carcinoma (DTC), total thyroidectomy, Tg<0.2 ng/ml; 2) without DTC and positive TPO; 3) without DTC and negative TPO. ATGs were measured by Immulite®, Cobas®, Architect® and Advia®. ATG’s classification was based on the cut-off of each manufacturer. Q-Chrocan test was used to calculate the p-values (P<0.05 were considered statistically significant).
Results: A total of 141 samples were analysed. The results are described in the table below: high significant differences were found between the various methods in all groups. The Architect and the Immulite had the highest and lowest rate of ATG positives, respectively, compared to Unicap.
| Positive ATG | Q test | |||||||
| Group | N | Unicap | Immulite | Cobas | Architect | Advia | χ2 | P |
| (A) DTC+/Tg<0.2 | 88 | 0 | 1 (1%) | 3 (3%) | 23 (26%) | 6 (7%) | 70.0 | <0.001 |
| (B) DTC−/TPO+ | 28 | 0 | 2 (7%) | 8 (29%) | 26 (93%) | 11 (39%) | 67.2 | <0.001 |
| (C) DTC−/TPO− | 25 | 0 | 0 | 1 (4%) | 6 (24%) | 2 (8%) | 17.7 | 0.001 |
| Total | 141 | 0 | 3 (2%) | 12 (9%) | 55 (39%) | 19 (14%) | 152.7 | <0.001 |
Discussion: ATG results depend on the equipment used. This can influence the follow-up strategy of patients with DTC. These results also raise the question of which method is more reliable for ATG detection and how a result of ‘ATG positive’ actually influences the Tg measurement.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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