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Endocrine Abstracts (2017) 49 EP1260 | DOI: 10.1530/endoabs.49.EP1260

ECE2017 Eposter Presentations: Thyroid Thyroid (non-cancer) (260 abstracts)

Non-thyroidal illness syndrome in chronic renal failure and oxidative stress: preliminary data on triiodothyronine relationships with extracellular superoxide-dismutase

Antonio Mancini 1 , Nicola Panocchia 2 , Giuseppe Martino 1 , Carmine Bruno 1 , Sonia Silvestri 3 , Patrick Orlando 3 , Fabio Marcheggiani 3 , Luigi Tazza 2 , Luca Tiano 3 , Giovanni Gambaro 2 & Alfredo Pontecorvi 1


1Catholic University of the Sacred Heart, Operative Unit of Endocrinology, Rome, Italy; 2Fondazione Policlinico Gemelli, Nephrology Division, Rome, Italy; 3Polytechnic University of Marche, Department of Life and Environmental Sciences, Ancona, Italy.


: Non-thyroidal-illness syndrome (NTIS) is present in chronic renal failure and considered an adaptive mechanism. However oxidative stress is linked to NTIS in a vicious circle, due to deiodinases alteration and negative effect on antioxidant levels or activity. One key antioxidant, protective toward ROS-mediated tissue damage, is extracellular superoxide-dismutase (ec-SOD), present in extracellular matrix and with a role in mediating nitric oxide-induced signaling. ec-SOD is specifically released from endothelium by heparin injection, allowing the determination of ec-SOD activity in vivo without affecting Cu,Zn-SOD or Mn-SOD. No data are reported on ec-SOD in renal failure and its relationship with thyroid function. Therefore we have studied 12 hemodialysis patients (9 males and 3 females, mean age 67 ys), evaluating thyroid hormones and ec-SOD activity after enoxaparin administration. Blood samples were obtained at the starting of hemodialytic session, 5 and 10 min after 1000–4000 U enoxaparin and at the end of the session. SOD activity was measured by a modified nitrite method. Superoxide generated by hypoxanthine and xanthine oxidase was changed by hydroxylamine to nitrite ion which was measured spectrophotometrically at 550 nm by the use of a chromogen. The amount of SOD required to inhibit 50% nitrite ion generation was defined as 1 U SOD activity. Six patients exhibited low fT3 values (1.8±0.1 pg/ml); the others had normal fT3 (2.5±0.2 pg/ml); fT4 and TSH values were normal. Basal eC-SOD did not differ between the two groups, but the percentual increase after heparin was significantly correlated with T3 levels (r=0.24, P<0.05). These preliminary data suggest that low T3 can negatively influence endothelial antioxidant defenses. The relationships of this datum with cardiovascular complications and prognostic usefulness remain to be established.

Volume 49

19th European Congress of Endocrinology

Lisbon, Portugal
20 May 2017 - 23 May 2017

European Society of Endocrinology 

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