Endocrine Abstracts

The biological organisms are complex systems regulated by the homeostatic back-end network. In these days the growing environmental loads (from industry, household, etc.) contain chemical exposure eg. halogenated hydrocarbons (monuron: MU (C₉H₁₁ClN₂O), diuron: DU (C₉H₁₀Cl₂N₂O)), which can be endocrine disruptor compounds (EDCs). Synthesis, release of hormones and cell processes of endocrine cells, and the biochemical properties of hormones can be changed by these agents. The disturbance of endocrine regulation by the EDCs can induce different diseases for example in the reproductive-, neurological, and/or psychical, and/or immunological system. In this study the aim was to investigate the effects of uron herbicides (fenuron: PU (C₉H₁₂N₂O); MU, DU) on the monoamine activated ocytocin (OT) release from neurohypophysis (NH) cells. The primary monolayer NH cell cultures were prepared from the evidenced Wistar rats (♂) for the experiments. The separated NH tissues were dissociated by enzymatic (trypsine, collagenase, DNA-se I; II) and mechanic methods. The 14 days old cultures were standardized for cell-viability and OT content. The NH cells were tested for functionally OT hormone volume by aspecific stimulus (30 mM [K⁺]). The untreated cultures were the controls, and the treating system was the consequential: A: 1 hour 0.1 μg/ml EDCs; B: 10^-6M monoamines (epinephrine, norepinephrine, dopamine, histamine, serotonine) alone; C: combined the monoamines agents with EDCs. The OT content was measured from supernatant media by RIA method. The results showed that the uron herbicides alone did not have significant effects on the OT release in the primary monolayer NH cell cultures. The EDCs caused alteration of the monoamine activated hormone release. The uron herbicides can play as strong environmental stress factors in the homeostatic system regulation. This work was supported by: TÁMOP-4.2.2.D-15/1/KONV-2015-0010, TÁMOP-4.2.4.A/2-11-1-2012-0001.