Endocrine Abstracts

Introduction: Obesity and diabetes mellitus type 2 (T2DM) are considered as one of the major risk factor for coronary heart disease (CHD) development, but the mechanisms beyond this relationship still need to be clarified. miRNAs are supposed to be involved in regulatory mechanisms in this relationship.

Aim: To determine miRNAs expression in patients with obesity and T2DM.

Methods: MiRNAs were detected in peripheral blood of 43 patients, aged 48–65 years with obesity and T2DM. Patients were divided in two groups: 1^st group – 21 patients with CHD, 2^nd group – 22 patients with excluded CHD after Tredmil-Test. All patients from the 1^st group underwent coronary angiography (CAG).

Results: Nine CHD-related mRNAs were selected as candidates for the investigation: miRNA-1, miRNA-21, miRNA-26a, miRNA-27a, miRNA-33a, miRNA-33b, miRNA-133a, miRNA-133b and miRNA-208. Expressions of these miRNAs were compared in studied groups. Expression of miRNA-21 (3.44 vs. 0.37; P=0.007) and miRNA-27a (0.86 vs.0.31; P=0.013) in patients with CHD were higher than that in 2^nd group, while expression of miRNA-26a (1.06 vs. 2.14; P=0.022) was lower in patients with CHD. Spearman correlation analyses showed that miRNA26a was positively associated with cholesterol level (r=0.316, P=0.042). MiRNA-21 and miRNA-27a were negatively correlated with HbA_1c in both groups (r=−0.363, P=0.042 and r=−0.420, P=0.036, respectively). MiRNA-208 was positively correlated with hypertrophy of the interventricular septum (r=0.61, P=0.027) and number of significantly affected coronary vessels according to CAG (r=0.65, P=0.034), negatively – with left ventricular diastolic dimension (r=−0.52, P=0.029).

Conclusion: 1) Expression of miRNA-21 was increased by 9 times, miRNA-27a in 2.5 times in patients with CHD, while miRNA26a compared with patients with type 2 diabetes and obesity was lower in 2 times.

2) We revealed correlations of miRNA-208, 27a, 21 with pathological angiogenesis, and heart remodeling processes in patients with obesity and T2DM.

3) Increased expression of miRNA-208 can determine the severity of cardiovascular disease in patients with obesity and T2DM.