ECE2017 Eposter Presentations: Diabetes, Obesity and Metabolism Diabetes complications (102 abstracts)
A protective effect of lobeglitazone on renal fibrosis in unilateral ureteral obstruction mice model
Hye-Soon Kim 1 , Kwi Hyun Bae 1 , Gwon Soo Jung 1 , Hye Jin Ham 1 , Bo Yoon Park 1 , Yeon Kyung Choi 1 , hyun Ae Seo 2 , Jung Beom Seo 1 & Keun Gyu Park 1
1Kyungpook National University Hospital, Dae Gu City, Republic of Korea; 2Fatima Hospital, Dae Gu City, Republic of Korea.
Renal tubulointerstitial fibrosis is a common feature of the final stage of nearly all cause types of chronic kidney disease. Although classic peroxisome proliferator-activated receptor-gamma (PPARγ) agonists have a protective effect on diabetic nephropathy, much less is known about their direct effects in renal fibrosis. This study aimed to investigate possible beneficial effects of lobeglitazone, a novel PPARγ agonist, on renal fibrosis in mice with unilateral ureteral obstruction (UUO). Through hematoxylin/eosin and Sirius red staining, we observed that lobeglitazone effectively attenuates UUO-induced renal atrophy and fibrosis. Immunohistochemical analysis in conjunction with quantitative RT-PCR and western blot analysis revealed that lobeglitazone treatment inhibited UUO-induced upregulation of renal Smad-3 phosphorylation, alpha-smooth muscle actin, plasminogen activator inhibitor-1, and type 1 collagen. In vitro experiments with rat mesangial cells and NRK-49F renal fibroblast cells suggested that the effects of lobeglitazone on UUO-induced renal fibrosis are mediated by inhibition of the TGF-β/Smad signaling pathway. In conclusion, the present study demonstrates that lobeglitazone has a protective effect on UUO-induced renal fibrosis, suggesting that its clinical applications could extend to the treatment of non-diabetic origin renal disease.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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