ECE2017 Eposter Presentations: Calcium and Bone Calcium & Vitamin D metabolism (65 abstracts)
The effect of 1,25(OH)2D3 on interferon i secretion by human mononuclear cells in vitro
Lambros Athanassiou 1 , Andrianos Nezos 1 , Ifigenia Kostoglou-Athanassiou 2 , Clio Mavragani 1 , Panagiotis Athanassiou 3 & Michael Koutsilieris 1
1Department of Physiology, Medical School, University of Athens, Athens, Greece; 2Department of Endocrinology, Red Cross Hospital, Athens, Greece; 3Department of Rheumatology, St Paul’s Hospital, Thessaloniki, Greece.
Vitamin D is a secosteroid hormone known for its skeletal effects. Recently, the extraskeletal effects of vitamin D are under investigation. Vitamin D is thought to exert an immunomodulatory effect, having multiple effects on the immune system. It is known to induce immune tolerance and also to enhance the immune response to bacteria.
The aim was to study the effect of 1,25(OH)2D3 on interferon I secretion by human mononuclear cells in vitro.
Human mononuclear cells were separated from whole human blood from healthy female human subjects using the Lymphoprep protocol. Subsequently they were placed in wells 106 cells/well and were cultivated for a period of 6 h at a temperature of 37 °C in a humidified environment of 5% CO2 in the presence or absence of interferon alpha 400 U, 1,25(OH)2D3 250 pmol, interferon alpha 400 U and 1,25(OH)2D3 250 pmol. Subsequently the content of the wells was centrifuged and the precipitate was treated with Trizol for RNA extraction of genes known to be stimulated by interferon alpha. Real time PCR was performed for MX1, IFIT1, IFI44 and GAPDH as a control.
Interferon alpha was shown to stimulate genes related to interferon I secretion, i.e. it was found to have a positive feedback on its own secretion. 1,25(OH)2D3 was found to modulate interferon I gene augmentation induced by interferon alpha treatment.
Vitamin D is being investigated for its immunomodulatory properties. In the present study vitamin D was found to modulate the response of human mononuclear cells to interferon alpha treatment.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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