ECE2017 Eposter Presentations: Adrenal and Neuroendocrine Tumours Endocrine tumours and neoplasia (50 abstracts)
Laboratory of Biochemistry and Immunology, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
Breast cancers that are estrogen receptor (ER)/progesterone receptor (PR)-positive are more likely to respond to hormone-related treatments than tumors that are ER/PR-negative. The present study investigated the effect of progesterone (P4) on 17β-estradiol (E2)-induced cell proliferation, apoptosis, EMT, and migratory and invasive features of MCF-7 clonal varient (CV) breast cancer cells that are ER/PR-positive. Preferentially, E2 was verified to induce breast cancer progression by stimulating cell proliferation, EMT, and migration of MCF-7 CV breast cancer cells. On the other hand, P4 reduced E2-induced MCF-7 CV cell proliferation by down-regulating the protein expression of cyclin D1 and E1 and induced apoptosis of MCF-7 CV cells by up-regulating Bax and p53 and by down-regulating Bcl-2. Also, P4 appeared to inhibit E2-induced EMT process by increasing mRNA and protein expression of E-cadherin, a crucial epithelial marker, as well as by reducing the expression of mesenchymal markers such as N-cadherin and vimentin and EMT-associated transcription factors such as snail and slug. Eventually, E2-induced migration and invasion ability of MCF-7 CV cells and the protein expression of proteolytic enzymes such as MMP-9 and cathepsin B were reduced by P4 treatment. Co-treatment of RU486, a PR inhibitor, restored the inhibited cellular migration and invasion and the reduced expression of proteases by P4 to the control levels, suggesting the involvement of PR in P4-induced inhibition on migration and invasion of MCF-7 CV cells. Taken together, P4 treatment may be suggested as an effective tool for suppression of human breast cancer progression and metastasis. (This work was supported by a grant from the Next-Generation BioGreen 21 Program (no. PJ011355-2015), Rural Development Administration, Republic of Korea.)
Keywords: Progesterone; breast cancer cells; epithelial-mesenchymal transition; metastasis; migration.