SFEBES2016 Poster Presentations Thyroid (26 abstracts)
High tumoral expression of PBF and PTTG modulates the DNA damage response and is associated with poor survival in thyroid cancer
Martin Read 1 , Jim Fong 1 , Waraporn Imruetaicharoenchoke 1 , Hannah Nieto 1 , Bhavika Modasia 1 , Alice Fletcher 1 , Rebecca Thompson 1 , Neil Sharma 1, , Andrea Bacon 3 , John Watkinson 1, , Kristien Boelaert 1 , Andrew Turnell 2 , Vicki Smith 1 & Christopher McCabe 1
1Institute of Metabolism and Systems Research, University of Birmingham, Birmingham, UK; 2Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, UK; 3School of Immunity and Infection, University of Birmingham, Birmingham, UK; 4University Hospitals Birmingham National Health Service Foundation Trust, Birmingham, UK.
Despite extensive genomic profiling a better understanding of the contributory factors that promote aggressive thyroid cancer is urgently needed. The proto-oncogenes PBF and PTTG have been implicated in thyroid cancer but there is a lack of information regarding their co-expression and specific roles in tumour progression. Separate studies have previously indicated that PBF and PTTG may disrupt pathways associated with the tumour suppressor p53 that are central to DNA-damage repair (DDR), cell growth and apoptosis. To further investigate this, we examined the association of PBF and PTTG with p53-related genes in the human thyroid TCGA cancer dataset, as well as in a bi-transgenic murine model (Bi-Tg) overexpressing PBF and PTTG specifically in the thyroid gland.
Characterisation of primary murine Bi-Tg thyrocytes revealed that co-expression of PBF and PTTG caused extensive repression of DDR genes (31/82 genes; >1.5-fold; P<0.05), including genes with key roles in maintaining genomic integrity such as Brca1. Irradiation exposure to cause DNA damage gave further evidence of significant repression of DDR genes (n=82) between irradiated Bi-Tg and wild-type thyrocytes (P=2.4×10−4) that was greater than either PBF-Tg (P=1.5×10−3) or PTTG-Tg thyrocytes (P=NS). By comparison in the TCGA dataset, there were striking correlations with PBF and PTTG in well-characterised p53-related gene panels (P<0.05; 82–96 genes per panel; n=322 TCGA tumour samples). Importantly, nearly half of the DDR gene alterations in Bi-Tg thyrocytes were also present in TCGA comparing tumours with either low or high PBF/PTTG expression. Furthermore, the overall survival (P=1.91×10−5) and disease-free survival (P=4.9×10−5) was poorer for TCGA individuals with high tumoral PBF/PTTG expression and mutationally activated BRAF than for all other patients.
Together our study provides important insights into the role of PBF and PTTG in modulating p53-related genes to promote tumorigenesis. We also identify using PBF and PTTG together as a new clinical indicator for aggressive thyroid cancer.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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