ECE2016 Guided Posters Bone & Calcium Homeostasis (10 abstracts)
Bone-specific circulating miRNA profile changes over an 8-week repeated sprint training protocol
Giovanni Lombardi 1 , Veronica Sansoni 1 , Silvia Perego 1 , Gianluca Vernillo 2, , Mattia Bonzanni 4 , Giampiero Merati 2, , Andrea Barbuti 4, , Antonio La Torre 2 & Giuseppe Banfi 1,
1I.R.C.C.S. Istituto Ortopedico Galeazzi, Milano, Italy; 2Department of Biomedical Sciences for Health, University of Milano, Milano, Italy; 3University of Verona, CeRiSM, Research Center for Sport, Mountain and Health, Rovereto, Italy; 4Department of Biosciences, University of Milano, Milano, Italy; 5I.R.C.C.S. Fondazione Don Carlo Gnocchi, Milano, Italy; 6Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata (CIMMBA), University of Milano, Milano, Italy; 7Vita-Salute San Raffaele University, Milano, Italy.
Introduction: Although underlying mechanisms are largely unknown, bone metabolism is regulated by specific microRNAs and changes in their expression are associated with metabolic bone disorders. Exercise is a determinant of bone metabolism; however, different training protocols differently affect bone. Little is known about the effects of exercise on bone-specific miRNA expression.
We studied the effect of a 8-week high-intensity training protocol on circulating levels of selected miRNA regulating bone metabolism.
Methods: 18 male adults were assigned to one of two groups. One group (n=9) performed repeated sprint training (3 times/week, 18 maximal all-out 15 m sprints with 17 s of passive recovery); the other served as a control group (n=9). Blood samples were taken before the start of the protocol (T0) and after 4 (T1) and 8 weeks (T2). Plasma circulating miRNAs (miR21 5P, miR125b 5P, miR23a 3P, miR93 5P, miR100 5P) were evaluated by real-time PCR (normalized on miR425 5P); relative expression levels were calculated by the 2−ΔΔCT method. Changes over time where tested by repeated measure one-way ANOVA, while inter-group differences at each time-point were tested throughout t test.
Results: While in the control group the miRNA expression remained stable, in the trained group miR23a 3P was 1.4-fold downregulated, between T0 and T2 (P<0.05). miR100 5P was 3-fold downregulated between T0 and T1 (P<0.05) and twofold downregulated between T0 and T2 (P<0.05). miR125b 5P expression was twofold downregulated at T1 compared to both T0 (P<0.01) and T2 (P<0.05).
Conclusions: An 8-week long high-intensity training protocol downregulates the expression of circulating miRNA associated with fracture risk.
Volume 41
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Endocrine Abstracts
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