ECE2016 Eposter Presentations Thyroid (non-cancer) (120 abstracts)
Immunohistochemical expression of coagulation and fibrinolysis markers in thyroid goiter
Łukasz Mielańczyk 1 , Oliwia Segiet 1 , Mariusz Deska 2 , Grzegorz Buła 2 , Adam Piecuch 1 , Grażyna Kowalczyk-Ziomek 1 & Romuald Wojnicz 1
1Medical University of Silesia, School of Medicine with the Division of Dentistry, Chair and Department of Histology and Embryology, Zabrze, Poland; 2Medical University of Silesia, School of Medicine with the Division of Dentistry, Chair and Clinical Department of General Surgery, Bytom, Poland.
Introduction: Endocrine disorders can influence the hemostatic balance. Various abnormalities of coagulation-fibrinolytic system have been reported in patients with thyroid dysfunction. The aim of the study was to assess the immunohistochemical expression and distribution of coagulation and fibrinolysis markers in follicular cells of thyroid goiter.
Materials and methods: 20 patients with thyroid goiter were included in the study: 11 cases with non-toxic nodular goiter (NTNG) and 9 patients with Hashimoto’s thyroiditis (HT). Normal glands served as controls. In a standard immunohistochemical procedure, monoclonal antibodies anti- tissue factor (anti-TF), anti-tissue factor pathway inhibitor (anti-TFPI), anti-von Willebrand factor (anti-vWF), anti-thrombomodulin (anti-TM), anti-tissue plasminogen activator (anti-tPA), anti-plasminogen activator inhibitor-1 (anti-PAI-1) and anti-D-dimers (anti-DD) were applied. The percentage of positively stained cells were counted and expressed as a mean value of at least 10 high power fields (400×).
Results: 80% of thyroid goiters demonstrated increased immunoreactivity for TM, 56% of cases showed up-regulated staining for vWF, 33% of thyroid tissues showed higher expression of TF, 25% of thyroids revealed enhanced immunopositivity for DD. In only 10% of goiters overexpression of both TFPI and tPA was found. PAI-1 level was unaltered in all of the examined thyroid glands. None of the healthy controls stained positively for these markers.
Conclusions: Abnormalities in the processes of coagulation and fibrinolysis are implicated in the pathogenesis of thyroid diseases, such as Hashimoto’s thyroiditis.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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