ECE2016 Eposter Presentations Thyroid cancer (81 abstracts)
Analysis of BRAF and RAS genetic alterations in thyroid cancer in the Greek population
Ifigenia Kostoglou-Athanassiou 1 , Marilena Argyropoulou 2 , Pagona Karanatsiou 2 , Georgios Papoutsidakis 2 , Alexandra Papadopoulou 2 , Kalliopi Loukou 2 , Aikaterini Manolakelli 2 , Eleni Synadaki 2 , George Vilaras 3 , Andreas Karameris 3 & Kalliopi Liadaki 2
1Department of Endocrinology, Red Cross Hospital, Athens, Greece; 2Department of Biochemistry and Biotechnology, Univeristy of Thessaly, Larissa, Greece; 3Department of Pathology, 417 Veterans Administration Hospital, N.I.M.T.S., Athens, Greece.
Introduction: Thyroid cancer is one of the most common malignancies of the endocrine system and displays a variety of histological patterns. The understanding of the molecular pathogenesis and the identification of molecular markers which will be used for diagnosis and prognosis is of high clinical significance. The most common molecular alterations include BRAF and RAS point mutations and RET/PTC and PAX8/PPARγ rearrangements. The present study investigated the association of BRAF and RAS mutations with thyroid cancer in a representative sample of the Greek population.
Methods: The study included 65 patients: 54 with Papillary Thyroid Cancer (PTC), 7 with Follicular, 3 with Medullary and 1 with Low Differentiation Thyroid Cancer. Following the isolation of genomic DNA from tissue biopsies a) real-time Polymerase Chain Reaction (PCR) and b) PCR and sequencing were used for the identification of mutations in codon 600 of the BRAF gene and in codons 12, 13 and 61 of the HRAS, KRAS and NRAS genes.
Results: BRAF mutations were identified in 8 PTC samples, half of which were of follicular subtype. All mutations include a 1799T→A conversion and a valine to glutamic acid substitution at codon 600. A PTC of follicular subtype was identified harboring a mutation in the NRAS gene (181C→A, resulting in a glutamine to lysine change in codon 61). Both mutations result in the activation of the MAP kinase signaling pathway. No mutations were identified in the specific codons of KRAS and HRAS genes.
Conclusion: Although the sample number is relatively small, the significantly low percentages of BRAF and RAS mutations point to the conclusion that the molecular alterations leading to thyroid cancer in the Greek Population may differ compared to those previously reported, and this consideration should be taken into account regarding the pathogenesis, progression and treatment of thyroid cancer.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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