ECE2016 Eposter Presentations Pituitary - Basic (17 abstracts)
HMGA2 as new biomarker of pituitary adenomas invasiveness?
Bruno Almeida Nunes 1, , Sérgio Filipe Portovedo 1, , Fernanda Oliveira Graça 1, , Gilvan Nascimento 4 , Nathalie Henriques Canedo 5 , Paula Soares 1, , Denise Pires de Carvalho 1, , Christina Maeda Takiya 3 , Manuel dos Santos Faria 1, & Leandro Miranda-Alves 1,
1Grupo de Pesquisa em Endocrinologia Experimental-GPEEx, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; 2Pós-graduação em Endocrinologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; 3Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; 4Faculdade de Medicina, Universidade Federal do Maranhão, São Luis do Maranhão, Brazil; 5Faculdade de Medicina, Serviço de Anatomia Patológica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; 6Cancer Biology Group, Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, Portugal.
Proper classification of pituitary adenomas (PA) is an important area of research. Thus, developing of new biomarkers is essential to facilitate the early detection of PA, identify the drug response and predict recurrence reliably in all patients. The usual histological markers of tumor aggressiveness and/or invasiveness are often similar between recurring, regrowing, and stable tumors, and therefore are not reliable as prognostic parameters. To quantitate the levels of the HMGA2 gene expression we analysed 41 PA samples for the specific HMGA2 mRNA levels by a qPCR assay. We are evaluated 12 Somatotropinomas, 20 NFPA, 7 Corticotropinomas, 1 Gonadotropinoma and 1 Prolactinoma. Based on preoperative tomography computerized (TC) images the PA were classified as invasive or non-invasive. Aggressiveness is defined by clinical behaviour and tumours were considered aggressive when the Ki-67 index was ≥ 3%. Here, we show by qPCR that HMGA2 mRNA expression significantly correlates with invasiveness. HMGA2 expression levels were higher in non-invasive PA (*P=0.0154) and significantly higher in non-invasive NFPA (**P=0.0087). In addiction, we evaluated the possible relationship of Ki-67 labeling index with HMGA2 expression as a predictor of tumor prognostic. No significant association was found.
Keywords: Pituitary Adenoma, HMGA2, Invasiveness, Ki-67, Aggressiveness
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more