ECE2016 Eposter Presentations Diabetes (to include epidemiology, pathophysiology) (83 abstracts)
Gut microbiota and diet in patients with various glucose tolerance
Lilit Egshatyan 1, , Daria Kashtanova 1 , Anna Popenko 2 , Olga Tkacheva 1 , Alexander Tyakht 2 , Dmitry Alexeev 2 , Natalia Karamnova 1 & Sergey Boytsov 1
1FSI ‘National Research Center for Preventive Medicine’ of the Ministry Healthcare of the Russian Federation, Moscow, Russia; 2Institute for Physical-Chemical Medicine, Moscow, Russia; 3Moscow State University of Medicine and Dentistry named after A.I. Evdokimov, Moscow, Russia.
Background: Type 2 diabetes (T2D) is a progressive disease. The gut microbiota has recently been identified as a new potential diabetes risk factor.
Design: To investigate the gut microbiota composition in association with the dietary patterns in patients with different glucose tolerance we analyzed 92 patients: with normal glucose tolerance (n=48), prediabetes (n=24) and T2D (n=20). Metagenomic analysis was performed using 16SrRNA sequencing. The diet has been studied by a frequency method with a quantitative evaluation of food intake using a computer program.
Results: Patients with different glucose tolerance did not differ among themselves in the amount of consumed proteins, fats, carbohydrates. Patients with various glucose metabolism disorders differed among themselves the level of fasting glucose and HbA1c (higher in T2D), as well as the energy value of the daily diet and the amount of consumed carbohydrates (less in T2D). Microbiota in the samples was predominantly represented by Firmicutes and in a less degree by Bacteroidetes. Blautia was a dominanting genus in all samples. The representation of Blautia, Serratia was lower in prediabetes than in T2D patients, and even lower in those with normal glucose tolerance. After the clustering of the samples into groups according to the percentage of protein, fat, carbohydrates in the diet, the representation of the Bacteroides turned to be lower and Prevotella abundance turned to be higher in carbohydrate cluster. There were more patients with T2D (P=0.002) in the fat-protein cluster. Using the Calinski-Harabasz index identified the samples with more similar diets. It was discovered that half of the patients with a high-fat diet had normal tolerance, the others had T2D. The regression analysis showed that these T2D patients also had a higher representation of Blautia (P=0.0001).
Conclusions: The high Blautia representation in combination with high-fat diet (138±63 g/day) is correlated with T2D.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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