ECE2015 Oral Communications Diabetes (5 abstracts)
Islet stellate cell isolated from Goto-Kakizaki rats affects β-cell function
Zilin Sun 1 , Fengfei Li 1, , Bijun Chen 1 , Ling Li 1 , Min Zha 1 , Wei Xu 1 , Shaoxia Zhou 3 & M G Bachem 3
1Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, Medical School, Southeast University, Nanjing, China; 2Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China; 3Department of Clinical Chemistry, University Hospital Ulm, Ulm, Germany.
Background: Evidence suggests that pancreatic stellate cells (PSCs) are present in intra-/peri-islets in type 2 diabetes mellitus (T2DM). We isolated stellate cell from islets (ISC) of Goto-Kakizaki (GK) rats. Given these cells are intra-islets, which possibly affect the biological behavior of β-cell. This study was designed to determine the effects of ISC isolated from fibrotic islets of GK rats on β-cell function and survival.
Methods: ISC was isolated from islets of GK rats using the outgrowth method. We determined the genome-wide digital gene expression (DGE) profiles of ISC by DGE technology. Insulin-producing β-cell line were treated with ISC supernatant (SN). Then, INS-1 cell proliferation, apoptosis, and insulin production were measured.
Results: ISC grew out from cultured GK rat islets, with a triangular shape and large nucleus. These cells expressed α-smooth muscle actin (α-SMA), vimentin, and desmin. ISC genes expression was significantly overlapped with that of PSC. We also observed 1067 genes differentially expressed in ISC compared with PSC. The 5-ethynyl-2′deoxyuridine-positive INS-1 cells treated with ISC-SN were significantly decreased compared with control. Propidium iodide (PI)-positive INS-1 cells in ISC-SN groups were 2.6-fold higher than those in control groups. Caspase-3 activity was associated with increased PI-positive cells. INS-1 cells cultured with ISC-SN were significantly reduced than that in control groups.
Conclusions: ISC presented in fibrotic islet of GK rats might be a special PSC, which impaired β-cell function, proliferation and increased β-cell apoptosis.
Disclosure: National Nature Science Foundation of China (nos 30971399, 81170716, and 81270010).
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more