ECE2015 Guided Posters Developmental and paediatric endocrinology (10 abstracts)
1Endocrine Laboratory, Department of Clinical Chemistry, VU University Medical Center, Amsterdam, The Netherlands; 2Endocrinology Unit, Department of Medical and Surgical Sciences, Center for Applied Biomedical Research, S. Orsola Malpighi Hospital, University of Bologna, Bologna, Italy; 3Department of Clinical Biochemistry, University Hospital of South Manchester, Manchester, UK; 4Biocrates Life Sciences AG, Innsbruck, Austria; 5Department of Pathology, ARUP Institute for Clinical and Experimental Pathology, University of Utah, Salt Lake City, Utah, USA; 6School of Clinical and Experimental Medicine, Centre for Endocrinology, Diabetes and Metabolism, University of Birmingham, Birmingham, UK; 7Department of Growth and Reproduction, Faculty of Health and Medical Sciences, Rigshospitalet, Copenhagen University Hospital, University of Copenhagen, Copenhagen, Denmark.
Recently, liquid chromatographytandem mass spectrometry (LCMS/MS) was stated to be the method of choice to measure sex steroids.1 Information on the mutual agreement of LCMS/MS methods, however, is scarce. Therefore, we compared seven published LCMS/MS methods for the simultaneous measurement of testosterone, androstenedione, and DHEA. Fifty-five random serum samples obtained from volunteers were analysed in duplicate by seven published LCMS/MS methods.2,3,4,5,6,7 We performed a PassingBablok regression analysis and calculated a Pearsons correlation coefficient to assess the agreement of the investigated methods with one of the methods in this study, arbitrarily chosen as reference method. Moreover, we calculated the intra-assay coefficient of variation (CV) of each method using the duplicate results. Concentrations of testosterone, androstenedione, and DHEA were 0.229, 0.45.2, and 1.818 nmol/l respectively. The slopes of the regression lines calculated by PassingBablok regression analysis ranged from 0.921.05 to 1.011.15 for testosterone values, for the entire data set and testosterone for concentrations below 2 nmol/l respectively. For androstenedione and DHEA the slopes were 0.961.28 and 0.961.46 respectively. The correlation coefficients ranged 0.9870.997, 0.9260.988, 0.9250.971, and 0.9550.988 for all testosterone values, testosterone concentrations below 2 nmol/l, androstenedione, and DHEA respectively. The intra-assay CV were 1.26.2, 2.910, 2.76.9, and 4.316% for testosterone values higher than 2 nmol/l, testosterone concentrations below 2 nmol/l, androstenedione, and DHEA respectively. In conclusion, in general the investigated LCMS/MS methods for simultaneous measurement of testosterone, androstenedione, and DHEA, showed a good agreement. However, there appear to be differences in standardisation between some of the assays and a high variation in some of the assays.
References: 1. Handelsman DJ et al. J Clin Endocrinol Metab 2013.
2. Büttler RM et al. Clin Chim Acta 2014.
3. Fanelli F et al. Steroids 2011.
4. Koal T et al. J Steroid Biochem Biol 2011.
5. Kushnir MM et al. Clin Chem 2010.
6. OReilly MW et al. J Clin Endocrinol Metab 2014.
7. Soeborg T et al. Clin Chim Acta 2013.