Dynamic thiol/disulfide homeostasis in patients with autoimmune subclinical hypothyroidism

Ihsan Ates; Mustafa Altay; Fatma Meric Yilmaz; Canan Topcuoglu; Salim Neselioglu; Ozcan Erel; Nisbet Yilmaz

doi:10.1530/endoabs.37.EP943

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Endocrine Abstracts (2015) 37 EP943 | DOI: 10.1530/endoabs.37.EP943

ECE2015 Eposter Presentations Thyroid (non-cancer) (160 abstracts)

Dynamic thiol/disulfide homeostasis in patients with autoimmune subclinical hypothyroidism

Ihsan Ates ¹ , Mustafa Altay ¹ , Fatma Meric Yilmaz ^2, , Canan Topcuoglu ² , Salim Neselioglu ³ , Özcan Erel ³ & Nisbet Yilmaz ¹

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¹Department of Internal Medicine, Ankara Numune Education and Research Hospital, Ankara, Turkey; ²Department of Biochemistry, Ankara Numune Education and Research Hospital, Ankara, Turkey; ³Department of Biochemistry, Medical Faculty, Yildirim Beyazit University, Ankara, Turkey.

Objective: To investigate dynamic thiol/disulphide homeostasis in autoimmune subclinical hypothyroidism.

Materials and methods: 48 patients with newly diagnosed subclinical hypothyroidism due to Hashimoto’s thyroiditis and not yet on any treatment and 48 healthy subjects without any known disease were enrolled. Thiol/disulfide homeostasis (native thiol(-SH)–disulphide(-S-S-) exchanges) was measured in both groups with new method developed by Erel and Neselioglu. The half of the difference between total thiol (-SH+-S-S-) and -SH concentrations gave the -S-S- bond amount.

Results: In patients with subclinical hypothyroidism, -SH level and -SH/(-SH+-S-S-) ratio was found to be lower than that of the control group. -S-S- level (P=0.004), -S-S-/-SH (P=0.001), and -S-S-/(-SH+-S-S-) (P=0.001) ratio was higher in patients with subclinical hypothyroidism as compared to that of the control group. A positive correlation was found between anti-TPO and anti-Tg levels and -S-S-/-SH ve -S-S-/(-SH+-S-S-) levels while a negative correlation was found with -SH/(-SH+-S-S-) level.

Conclusions: Thiol/disulfide homeostasis was found to have a tendency towards -S-S- formation in patients with subclinical hypothyroidism and thyroid autoantibodies were correlated positively with thiol oxidation. Abnormal thiol/disulfide homeostasis in patients with Hashimoto’s thyroiditis, is whether a cause or a consequence, may be illustrated by using thiol-containing drugs and following autoantibody levels. The efficacy, dose and duration of thiol drugs may be monitored easily, effectively, quickly and cheaply by the method developed by Erel and Neselioglu.