Endocrine Abstracts

Introduction: Changing of expression of pattern-recognition receptors (PRR) of innate immune response by the gut-associated lymphoid tissue (GALT) can play a critical role in an induction and progression of T1DM.

Aim: To study the peculiarities of NOD2 receptors in gut-associated lymphoid tissues (GALT) of rats with experimental STZ-induced diabetes mellitus and pentoxifylline (PTX) administration.

Methods: Researches were made on Wistar rats. For an induction of diabetes streptozotocin was used in doses 50 mg/kg. Structure of population of NOD2+ cells has been studied by the analysis of serial histological sections using the method of indirect immunofluorescense with monoclonal antibodies to NOD2 of rat.

Results: It has been established that diabetes development was accompanied with 37–45% (P<0.05) increase in quantity of NOD2 cells on the 14th day, but by the 4th week of disease their number returned to the benchmarks. Induction of diabetes leads to increased in concentrations of NOD2 on 7–28% in macrophages and dendritic cells and a decrease on 8–12% in lymphocytes. PTX administration of diabetic animal reduces the quantity of NOD2+ cells on 29% (P<0.05) in mucous membrane of villus 42% in subepithelial zone of ILF by the 14th day of experimental diabetes mellitus. But by the 4th week of disease their number returned to the benchmarks in mucous membrane of villus and in subepithelial zone of ILF it increase on 29%. The concentration of NOD2 also decreased in 2nd week of diabetes on 8–15% in NOD2+-macrophages and NOD2+-dendritic cells.

Conclusions: The expression augmentation with NOD2 in ileum immunopositive cells can influence the differentiation of subsets of immunopositive cells and their proinflammatory cytokines production, thus acting as one of triggers of diabetes development and progression.