ECE2015 Eposter Presentations Endocrine tumours (69 abstracts)
Expression analysis of potentially MEN1-targeting microRNAs in sporadic and MEN-1 syndrome associated parathyroid adenomas and hyperplasias
Vince Kornél Grolmusz 1, , Katalin Borka 3 , Katalin Balogh 1 , Anna Szentpéteri 1 , Csaba Dékány 3 , András Kiss 3 , Zsuzsanna Valkusz 4 , Miklós Tóth 1 , Anikó Somogyi 1 , János Horányi 5 , Károly Rácz 1, & Attila Patócs 2,
12nd Department of Medicine, Semmelweis University, Budapest, Hungary; 2‘Lendület’ Hereditary Endocrine Tumours Research Group, Hungarian Academy of Sciences – Semmelweis University, Budapest, Hungary; 32nd Department of Pathology, Semmelweis University, Budapest, Hungary; 41st Department of Medicine, University of Szeged, Szeged, Hungary; 51st Department of Surgery, Semmelweis University, Budapest, Hungary; 6Molecular Medicine Research Group, Hungarian Academy of Sciences – Semmelweis University, Budapest, Hungary.
Background and aim: Primary hyperparathyroidism (PHPT) is a frequent endocrinopathy among postmenopausal women, leading to hypercalcaemia, osteoporosis and nephrolithiasis. PHPT may represent the first manifestation of certain familial syndromes. Among these, multiple endocrine neoplasia syndrome type 1 (MEN-1) caused by germline mutation of MEN1, the gene encoding menin, is the most frequent. Additionally, somatic mutations of MEN1 and microRNAs silencing MEN1 have also been proposed to be involved in sporadic parathyroid tumourigenesis. Our aim was to determine if there is a difference in expression of potentially MEN1-targeting microRNAs between MEN-1 syndrome associated and sporadic PHPT tissues.
Materials and methods: In silico analysis was performed to detect microRNAs potentially targeting MEN1. Immunohistochemical analysis of menin and Ki67 was performed in 16 MEN-1 associated and 41 sporadic PHPT tissues. RNA was isolated from formalin-fixed, paraffin-embedded PHPT tissues and microRNA expression analysis of six chosen microRNAs was performed using predesigned TaqMan probes for quantitative PCR. MEN1 status was determined by Sanger sequencing. Statistical analysis was performed using IBM SPSS Statistics Software.
Results: All MEN-1 associated as well as 12 (29.3%) sporadic PHPT tissues lacked nuclear menin expression on immunohistochemical analysis. MicroRNA analysis revealed that hsa-miR-24 and hsa-miR-28 levels were elevated in sporadic PHPT tissues compared to those measured in MEN-1 associated tissues (P=0.011 and P=0.019, respectively).
Conclusions: Elevated levels of microRNAs potentially targeting MEN1 detected in sporadic PHPT tissues might contribute to the silencing of menin during sporadic parathyroid tumourigenesis.
Acknowledgement: The authors acknowledge the financial support from Hungarian Scientific Research Fund (OTKA, PD100648 (AP)) and Technology Innovation Fund, National Developmental Agency (KTIA-AIK-2012-12-1-0010).
Disclosure: The authors acknowledge the financial support from Hungarian Scientific Research Fund (OTKA, PD100648 (AP)) and Technology Innovation Fund, National Developmental Agency (KTIA-AIK-2012-12-1-0010).
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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