ECE2015 Guided Posters Thyroid–genetics (8 abstracts)
Gene expression profiling in differentiated thyroid carcinoma
Sorina Schipor 1 , Ana-Maria Niculescu 3 , Dana Manda 1 , Andra Caragheorgheopol 1 , Sabina Oros 1 , Susana Vladoiu 1 , Oana Popa 1 , Diana Paun 1, & Corin Badiu 1,
1National Institute of Endocrinology C. I. Parhon, Bucharest, Romania; 2University of Medicine and Pharmacy Carol Davila, Bucharest, Romania; 3Agilrom Scientific SRL, Bucharest, Romania.
Thyroid carcinoma is the most common endocrine malignancy and represents ~1% of all types of human cancer.
Objective: As the molecular pathogenesis of thyroid cancer still remains to be clarified, the goal of our study was to find new molecular markers that could improve the diagnostics, follow-up protocols, treatment outcome, prognosis and the quality of life of differentiated thyroid cancer patients.
Subjects and methods: Matched tumour and normal thyroid tissue samples were obtained from patients that have surgical cure for differentiated thyroid carcinoma after they gave their informed consent. RNA was extracted using the RNeasy Mini Kit from Qiagen and the quality was checked with the Infinite® 200 NanoQuant (Tecan) and with the 2100 Bioanalyzer (Agilent). 24 samples with RIN >7 were chosen for microarray gene expression analysis (six with classical papillary thyroid carcinoma and six with papillary thyroid carcinoma folicullar variant). Microarray analysis was performed following Agilent One-Color Microarray-Based Gene Expression protocol, ver 6.6, using SurePrint G3 Human Gene Expression arrays 8x60K v2.
Results: Using GeneSpring ver 12, we identified 25 genes and two long intergenic non-protein coding RNA (lincRNA 1140 and BROAD Institute lincRNA (XLOC_005062)/lincRNA (TCONS_00010536) down regulated in the tumour tissue compared with the normal one, P value <0.05 and fold change ≥2. When accounting for the two thyroid cancer types studied, we identified three genes up-regulated (COL13A1, EDA2R, and KLHDC8A) and eight down regulated (SLITRK5, CCL21, TFPI, TBX1, LOC389033, ADH1C, MMRN1, and F10) in both subtypes. The level of gene expression disregulation is much higher in the case of classical papillary thyroid carcinoma.
Conclusion: Gene expression is altered in papillary thyroid carcinoma. Our study identified three hyper-expressed genes and eight genes with low expression in tumoural tissue compared to normal one. Further studies are undergoing for gene expression data validation by qPCR.
Disclosure: This work was supported by UEFISCDI (grant number PN-II-PT-PCCA-2011-3.2, 135/2012).
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Endocrine Abstracts
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