ECE2015 Eposter Presentations Pituitary: basic and neuroendocrinology (62 abstracts)
1Krasnoyarsk State Medical University named after Prof. V. F. Voino-Yasenetsky, Krasnoyarsk, Russia; 2Siberian Division, Institute for Medical Problems of the North, Russian Academy of Medical Sciences, Krasnoyarsk, Russia; 3Krasnoyarsk Regional Clinical Hospital, Krasnoyarsk, Russia.
Somatotropin (GH) and IGF1 realise their influence on the system of intracellular metabolism and a number of important biochemical lymphocytes reactions through the receptor apparatus. It is allows to use the peripheral blood lymphocytes as the investigations object of intracellular metabolism disorders in acromegaly.
Aim: To study the NAD- and NADP-dependent dehydrogenases activity in blood lymphocytes in active acromegaly patients.
Methods: The activity of NAD(P)-dependent dehydrogenases in blood lymphocytes was studied in a group of 88 patients (35 men and 53 women) with active acromegaly, mean age 51.0±12.5 years. The NAD(P)-dependent dehydrogenases activity was determined by biochemiluminescence method. The concentrations of GH and IGF1 were measured by ELISA.
Results: Studying the activity of mitochondrial NAD(P)-dependent dehydrogenases found a decrease in all NAD-dependent oxidoreductase: NADIDH, NADGDH, and MDH (P<0.01), which allows to state the low level flow in the tricarboxylic acid cycle. In active acromegaly were revealed the decreasing activity of all studied oxidoreductases: glucose-6-phosphate dehydrogenase (P<0.01), NADlactate dehydrogenase (LDH) (P<0.001), NADHLDH (P<0.001), NADmalate dehydrogenase (MDH) (P<0.001), NADHMDH (P<0.001), NADPMDH (P<0.001), NADglutamate dehydrogenases (GDH) and NADHGDH (P<0.001), NADPGDH and NADPHGDH (P<0.001), NADisocitrate dehydrogenases (IDH) and NADPIDH (P<0.01 and P<0.001 respectively), and glutathione reductase (P<0.001). Our data observed that decreasing activity of NADPGDH positively correlated with the basal GH level (r=+0.23, P=0.04) and NADPMDH activity with IGF1 level (r=+0.30, P=0.008). The low NADHMDH activity negatively correlated to the basal GH concentration (P=−0.23, P=0.04).
Conclusion: The chronic excess of GH and IGF1 in acromegaly causes a significant depletion of metabolic lymphocytes reserves. The main indicators of functional lymphocytes impairment in acromegaly are: the reduction of intermediates formation for the reactions of macromolecular synthesis and aerobic processes, the low intensity of glycolysis, nitrogen metabolism and inhibition of glutathione complex activity.