Endocrine Abstracts

In obesity the combined effects of enhanced food consumption, enhanced oxidative stress and inflammation could increase levels of advanced glycation end products (AGEs) and the action of their receptors.

Objective: To study circulating levels of AGEs, soluble RAGE and AGER-1 and their association with insulin resistance and inflammation in young subjects with obesity and normal weight.

Material and methods: We conducted a cross-sectional study in obese Young (n=80) and normal weight (n=80). We calculated the BMI according to Cole et al., and the consumption of AGEs in the diet (dAGEs) with the tables of Uribarri et al. We measured glucose, lipid profile, insulin, HOMA-IR, TNF-α, serum AGEs (CML) and sRAGE. In a subsample of 27 subjects with normal weight and 21 subject with obesity we also measured the expression of RAGE and AGER1 by qPCR.

Results: We studied a total of 160 subjects (16±1 years old) and 55% females. The group with obesity show higher levels of triglycerides (P<0.003), insulin (P<0.001), HOMA-IR (P<0.001) and TNF-α (P<0.04). In the groups with obesity and consumption of AGEs >9000 KU/day we found higher levels of insulin (P<0.0001), HOMA-IR (P<0.01) and sRAGE (P<0.04). CML was associated with HOMA-IR (P<0.02) and TNF-α (P<0.03). In the sample were we studied the expression of cellular receptors we found higher expression of AGER-1 in the obesity group (P<0.02) and association of AGER-1 with weight (P<0.02) and dAGE (P<0.02) and RAGE with physical activity (t-2.14; P<0.03).

Conclusion: The results show higher insulin resistance and inflammation in the subjects with obesity, also association of CML with insulin resistance and TNF-α; and higher expression of AGER1 in the obesity group.

Disclosure: Supported by University of Guanajuato DAIP 2013.