Endocrine Abstracts

Objective: The aim of our study was to investigate the relationships between insulin receptor substrat-1 (IRS-1) and insulin receptor substrat-2 (IRS-2) gene polymorphisms and cardiovascular factors such as blood pressures, BMI, LDL- cholesterol, triglyceride, HOMA-IR, homocysteine, hsCRP and fibrinogen levels in patients with metabolic syndrome (MetS).

Subjects and methods: The study population included 100 patients with MetS and 30 patients without MetS as control group. All entire coding exons of IRS-1 and IRS- 2 gene were amplified by PCR. Insulin resistance (IR) was estimated using the homeostasis model assessment (HOMA).

Results: In patients with MetS, 34 (34%) patients had G972R gene polymorphism and 66 (66%) had no nucleotide substitution at IRS-1 gene (P<0.0001). And, in IRS-2 gene, 44 (44%) had no nucletide substitution, 18 (18%) had G1057D heterozygous, 11 (11.0%) had G1057D homozygous, 2 (2%) had P1031P heterozygous/P1033PG1057 heterozygous, 17 (17.0%) had P1033P hererozygous, 3 (3.0%) had P1033P homozygous and 5 (5%) had P1033P heterozygous/G 1067D heterozygous polymorphisms in MetS (P=0.0001). However, all of control had no nucleotide substitution in IRS-1 and IRS-2 genes. There were no statistically differences between patients who had gene polymorphism and not polymorphism in IRS-1 and IRS-2 for systolic and diastolic blood pressures, BMI, LDL- cholesterol, triglyceride, HOMA-IR, homocysteine, hsCRP and fibrinogen.

Conclusion: Insulin receptor substrat 1 and 2 genes polymorphisms were associated with metabolic syndrome but not cardiovascular risk factors such as blood pressure, BMI, cholesterol, triglyceride, HOMA-IR, homocysteine, hsCRP and fibrinogen levels.