Endocrine Abstracts

Background: Various genetic and environmental factors are appear to contribute to the development of kidney disease in type 2 diabetes. Small ubiquitin-related modifier 4 (SUMO4) appear to play a role in development and/or progression of diabetic nephropathy. Substitution of methionine with valine at codon 55 (M55V) of SUMO4 enhances activity of nuclear factor-kB, a central mediator of inflammation proapoptotic events.

Aims: To investigate the association between the SUMO4 M55V (rs237025, 163G>A) variant and kidney disease in north Indian individuals with type 2 diabetes.

Materials and methods: A case–control analysis was performed using genomic DNA samples from 201 subjects with diabetic nephropathy (DN) and 216 patients with diabetes but no kidney disease (DM). The SUMO4 163G>A polymorphic region was amplified using PCR and genotyping was done by digestion of amplified PCR product with restriction enzyme MseI.

Results: The duration of diabetes was higher in DN compared to DM (P=0.001). Clinical characteristics like blood pressure, cholesterol, triglycerides, HbA1c, and serum creatinine were significantly different between the two groups (P<0.001). The frequency of other diabetic complication like neuropathy and retinopathy was also significantly greater in DN (P<0.001). The genotypic and allelic frequencies of the studies variant were significantly different in DM and DN groups. GG genotype and G allele were significantly more frequent in DN as compared to DM ((GG: P=0.018, OR=1.72, 95% CI 1.1–2.7) (G: P=0.017, OR=1.4, 95% CI 1.1–1.8)).

Conclusions: This study shows that SUMO4 163G>A polymorphism might be associated with susceptibility to diabetic nephropathy.