ECE2014 Poster Presentations Obesity (53 abstracts)
The mRNA levels of TNFα, Omentin-1 and PPARγ in adipose tissue of patients with abdominal obesity
Mikhail Nikolaev 1 , Tatiana Usenka 1, , Elena Bazhenova 2 , Xe Zhu 2 , Olga Listopad 2 , Alexandr Neimark 2 , Petr Bogdanov 2 , Olga Berkovich 2 , Elena Baranova 2 & Sofya Pchelina 1,
1Petersburg Nuclear Physics Institute, St-Petersburg, Russia; 2Pavlov’s State Medical University of Saint-Petersburg, St-Petersburg, Russia.
Adipose tissue is a complex, essential, and highly active metabolic and endocrine organ that release a large numbers of bioactive mediators (adipokines). Obesity is defined as an excessive growth of adipose tissue. As such, it is likely that adipokines could play an important role in the development of diseases associated with obesity including insulin resistance, inflammation, hypertension, cardiovascular risk and metabolic disorders. PPARγ is a ligand-activated transcription factors that is implicated in adipocyte differentiation, function and influence on adipokine gene expression.
The aim of our work was to estimate the TNFα, Omentin-1 gene, PPARγ mRNA levels in visceral fat of patients with abdominal obesity and controls.
We generated the visceral fat of 23 patients with abdominal obesity (mean age 45±8 years, 4 males, mean BMI 34.7±8.5) and 7 controls (mean age 42.1±10 years.8, 5 males, BMI 24.0±2.6). Visceral fat was received from gastrocolic omentum during laparoscopic cholecystectomy in non-acute period of gall-stone disease. TNFα, PPARγ, Omentin-1 gene mRNA levels were estimated by RT-PCR with TaqMan probes. G protein mRNA levels (GNB2L1) was used as internal control.
Difference in TNFα, Omentin-1 gene, PPARγ mRNA levels between patients with abdominal obesity and controls was not statistically significant (P>0.05). Spearman correlation analysis showed positive correlation between PPARγ mRNA levels and Omentin-1 mRNA levels (r=0.552; P=0.033) in patients with abdominal obesity in visceral fat.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more