Endocrine Abstracts

Diabetes mellitus (diabetes) can now be called the disease of modern times due to its increasing incidence especially in the more developed parts of the world. It is characterized by the inability of the body to produce and/or properly use insulin. In our work we engaged in research of type 1 diabetes in NOD animal model. The focus of our study was relatively recently discovered SGLT time in the small intestine, which are actively inhibited by Ca²⁺ zeolite. SGLT1 transporter is Na⁺/glucose koransporter located in the small intestine and the small amount in the kidney.

The mice are divided into three main groups: two control diabetic (diabetic who received insulin and diabetic receiving insulin+probe with Ca²⁺-zeolite). Animals were tested for the ability of current glucose tolerance test glucose tolerance. Then, in order to determine the direct effect of the application of the Ca²⁺-zeolite concentration of glucose at normal physiological conditions, the test animals were used to determine the concentration of glucose in the peripheral blood after feeding. Results in both cases showed significantly better glucose regulation in the group of diabetic mice which received the probe with Ca²⁺- zeolite compared to the diabetic group that received the physiological solution. We then incubating small intestine in vitro followed the Na/Ca exchange in order to check whether the activity goes beyond the presumed active glucose transport. How to track metabolic changes in the treated mice were placed for 24 h in the metabolic cages after 14-day of treatment. The results showed a clear difference in the amount of urine excreted between the two diabetic groups. Finally, to monitor the in vivo process of glucose, all three groups of animals were recorded and are microPET camera and the results clearly showed accumulation of glucose in animals treated with Ca²⁺-zeolite.

Results of this study clearly demonstrate the positive impact of the application of Ca²⁺-zeolite in combination with insulin in the regulation of hyperglycemia (and thus in the prevention of late complications).