ECE2014 Poster Presentations Thyroid Cancer (70 abstracts)
DNMT1 expression in papillary thyroid carcinoma
Andreea Brehar 1 , Camelia Procopiuc 1 , Diana Paun 1, , Dana Manda 1 , Sabina Oros 1 , Andra Caragheorgheopol 1 , Alexandra Bulgar 1 , Felix Brehar 2 , Cristina Dumitrescu 1 , Iuliana Gherlan 1 , Mircea Ghemigian 1 , Bogdan Stanescu 1 , Daniel Brasoveanu 1 , Dana Terzea 1, , Dumitru Ioachim 1 , Adriana Plesa 3 , Irina Huica 3 , Iulia Iancu 3 , Corin Badiu 1, & Constantin Dumitrache 1,
1C.I.Parhon National Institute of Endocrinology, Bucharest, Romania; 2University of Medicine and Pharmacy Carol Davila, Bucharest, Romania; 3Stefan S. Nicolau Institute of Virology, Bucharest, Romania.
Introduction: In human cancer cells DNMTs are responsible for both de novo and maintenance methylation of tumor suppressor genes. Many studies have analyzed the relationship between the altered expression of DNMT1 and DNA methylation in cancer.
Aim: To analyze DNMT1 expression in thyroid papillary carcinoma.
Design: 49 patients aged 10–82 years hospitalized for thyroidectomy were included between January 2013 and July 2013. The inclusion criterion was patients with thyroid nodules with indication for surgery and the exclusion criteria were: hyperthyroidism, medullary thyroid carcinoma, thyroid metastasis, other thyroid tumors, and anaplastic thyroid carcinoma. Patients were divided into three groups. Group 1: 26 subjects with papillary thyroid carcinoma. Group 2: 14 patients with follicular adenoma. Group 3: nine patients with multinodular goiter. Group 1 was divided in: Group 1.1- classical variant of papillary thyroid carcinoma, Group 1.2 follicular variant of papillary carcinoma; Group 1.3 diffuse sclerosing variant of papillary carcinoma. Total RNA was isolated from tissues and reverse transcribed. DNMT1 expression levels were investigated by qRT-PCR using Taqman (Applied Biosystem). Double normalization was performed related to gene expression levels found in peritumoral tissues. SPSS 18 (2010) program was used to perform statistical analyze. Results were compared using Kruskal–Wallis and Mann–Whitney U tests and were considered statistical relevant if P<0.05.
Results: DNMT1 gene expression in patients with papillary thyroid carcinoma was not increased comparing with controls (P>0.05). The comparison of cancer subtypes did not reveal significant differences for DNMT1. DNMT1 expression did not correlate with tumor stage, tumor multifocality, capsular, vascular or lymphatic invasion or with metastasis.
Conclusion: DNMT1 is not overexpressed in papillary thyroid carcinoma and does not correlate with tumor stage, tumor multifocality, capsular, vascular or lymphatic invasion or with metastasis.
Acknowledgements: Results are part of the research project PCCA2 nr. 135/2012.
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