ECE2014 Poster Presentations Thyroid Cancer (70 abstracts)
The role of podoplanin in the biology of differentiated thyroid cancers
Kamila Karpinska 1 , Magdalena Rudzinska 1 , Damian Gawel 1 , Miroslaw Kiedrowski 2 , Tomasz Stepien 3 , Magdalena Marchlewska 3 , Domek Hanna 1 & Barbara Czarnocka 1
1Department of Biochemistry and Molecular Biology, Center of Postgraduate Medical Education, Warsaw, Poland; 2Department of Pathology, Institute of Oncology, Maria Sklodowska-Curie Memorial Cancer Center, Warsaw, Poland; 3Department of General and Endocrinological Surgery, Copernicus Memorial Hospital, Lodz, Poland.
Podoplanin (PDPN), a mucine type transmembrane glycoprotein specific to lymphatic system is expressed in a variety of human tumors and regarded as a factor promoting tumor progression. In a recent work, we found PDPN expression in tumor cells of 40% of papillary thyroid carcinomas (PTC), however it role remains unclear. The purpose of this study was to elucidate the molecular role of PDPN in the biology of thyroid cancer cells.
PDPN gene and protein expression was analyzed in primary thyroid carcinomas and thyroid carcinoma cell lines (TPC1, BcPAP, FTC133, and CGTH-W-1) and NTHY-ori-3-1 cell line by quantitative RT-PCR, western blot, ICC and IHC. To examine podoplanin role in regulating the hallmark of malignant cell phenotype: proliferation, migration, invasion and adhesion of TPC1 cells siRNA silencing of PDPN were analyzed.
We observed that PDPN was solely expressed in cancer cells of 40% of thyroid tumor tissues. Moreover, PDPN mRNA and protein was highly expressed in PTC derived TPC1 and BCPAP, and significantly down-regulated in FTC derived cell lines. Knockdown of PDPN with sRNA significantly decreased cellular invasion, modestly reduced cell migration and motility, whereas proliferation and adhesion were not affected.
This is the first study to characterize the expression and function of PDPN in thyroid cancer. Our results demonstrate that PDPN mediates invasion in papillary thyroid carcinomas derived cell lines, suggesting that podoplanin might mediate thyroid papillary carcinoma progression.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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