ECE2014 Poster Presentations Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) (11 abstracts)
Correlation of coexpression of pituitary genes in QPCR and microarray study in pituitary adenomas
Jadwiga Zebracka-Gala 1 , Dawid Larysz 2 , Aleksandra Pfeifer 1 , Adam Rudnik 2 , Jolanta Krajewska 1 , Kornelia Hasse-Lazar 1 , Malgorzata Kowalska 1 , Piotr Bazowski 2 & Barbara Jarzab 1
1Department of Nuclear Medicine and Endocrine Oncology, MSC Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland; 2Department of Neurosurgery, Silesian University School of Medicine, Katowice, Poland.
Introduction: Mechanism of pathogenesis of pituitary adenomas is still unknown. Gene expression differences in pituitary cells of different origin are not extensively described. Identification of genes specific for pituitary adenomas should enable better understanding of differences in their response to therapy, especially to radiotherapy.
Aim: The aim of our study was to evaluate the correlation of coexpression of distinct pituitary adenoma genes based on QPCR and microarray study.
Material and methods: Analysis of gene expression was performed by QPCR in 76 pituitary adenomas, 25 functioning and 51 nonfunctioning ones. Expression of the examined genes was normalized to the reference index, obtained by calculation of geometric mean of reference genes expression: GUS-B, B2M, ACTB, EIF3S10, UBE2D2, and ATP6V1E.
Microarray study was performed with Illumina HumanRef-8 v3 microarrays (Illumina, Inc.) in 48 pituitary adenomas: 36 functioning (GH, PRL, and TSH) and 12 nonfunctioning ones (‘0’). Microarray analysis was performed by BRBArrayTools and R-Bioconductor.
Results: During the assessment of significant correlation between QPCR and microarray results for PRL and GH gene (R=0.93; R=0.94 P<0.001) an unexpected clustering of adenoma subtypes was noticed.
As expected, the highest level of expression for PRL gene was observed in prolactinomas and for GH gene in somatotropinomas respectively. GH expression was 100× higher in somatotropinomas than prolactinomas. When we compared QPCR and microarray results we observed that GH gene was virtually absent in ‘0’ adenomas. Expression of PRL gene was seen in somatotropinomas (4.2× lower in comparison to PRLomas).
Conclusions: The differences in gene expression profiles between pituitary adenomas are confirmed. However, the assessment using simultaneously QPCR and microarrays allowed to separate different subtypes of adenomas.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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