Different relationship between serum 25OH-vitamin D and expression of vitamin D 25-hydroxylase gene CYP2R1 in subcutaneous adipose tissue of obese and slim subjects.

Marta Jonas; Alina Kurylowicz; Zbigniew Bartoszewicz; Wojciech Lisik; Maurycy Jonas; Zbigniew Wierzbicki; Andrzej Chmura; Monika Puzianowska-Kuznicka

doi:10.1530/endoabs.35.P784

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Endocrine Abstracts (2014) 35 P784 | DOI: 10.1530/endoabs.35.P784

ECE2014 Poster Presentations Obesity (53 abstracts)

Different relationship between serum 25OH-vitamin D and expression of vitamin D 25-hydroxylase gene CYP2R1 in subcutaneous adipose tissue of obese and slim subjects.

Marta Jonas ¹ , Alina Kurylowicz ¹ , Zbigniew Bartoszewicz ^1, , Wojciech Lisik ² , Maurycy Jonas ² , Zbigniew Wierzbicki ² , Andrzej Chmura ² & Monika Puzianowska-Kuznicka ^1,

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¹Department of Human Epigenetics, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland; ²Department of Geriatrics and Gerontology, Medical Center of Postgraduate Education, Warsaw, Poland; ³Department of General and Transplantation Surgery, Medical University of Warsaw, Warsaw, Poland; ⁴Department of Internal Medicine and Endocrinology, Warsaw Medical University, Warsaw, Poland.

Introduction: In Central Europe, vitamin D deficiency is common, and a very pronounced deficiency of this vitamin is observed in obese individuals. We hypothesized that the decreased serum concentration of vitamin D might be in part due to the changed metabolism of this vitamin in adipose tissues.

Methods: We assessed the expression of vitamin D 25-hydroxylase genes CYP2J2 and CYP2R1 in adipose tissues of obese and slim patients using RT-PCR. Subcutaneous (SAT) and visceral (VAT) adipose tissues were obtained from obese subjects (n=62, BMI 39-68 kg/m²) during bariatric surgery (RYGB or VBG) and from non-obese controls (n=30, BMI 20-26 kg/m²) undergoing scheduled surgical procedures. Serum concentrations of 25OH-vitamin D were measured with the Diagnostics Vitamin D Total Assay (Roche) and IDS manual immunoassay. The results of both tests were correlated with each other.

Results: The mean serum concentration of 25OH-vitamin D was low and similar in obese and slim patients (15.23 ng/ml vs 18.97 ng/ml respectively, P>0.05). The mean expression of CYP2J2 was similar in VAT (107±20.01 arbitrary units (AU) vs 110±5.63 AU, P>0.05) and in SAT (112.80±8.66 AU vs 107.80±4.64 AU, P>0.05) of obese vs slim subjects. Similarly, the mean expression of CYP2R1 in VAT (120.19±12.43 AU vs 110.05±5.62 AU, P>0.05) and in SAT (119.60±12.60 vs 110.44±5.88 AU, P>0.05) was similar in obese vs slim subjects. A negative correlation (R=−0.30) between the expression of CYP2R1 in SAT of obese individuals and serum concentration of 25OH-vitamin D was found, while it was positive (R=0.20) in SAT of slim patients.

Conclusion: The expression of CYP2R1 is similar in VAT and SAT of obese and slim patients. Nevertheless, an opposite relationship between serum concentration of 25OH-vitamin D and the expression of this gene in SAT of obese and slim patients has been noted.