ECE2014 Poster Presentations Obesity (53 abstracts)
Higher serum levels of the Wnt-signaling antagonist DKK1 in obese respect to Prader–Willi syndrome
Giacomina Brunetti 1 , Maurizio Delvecchio 2 , Grugni Graziano 3 , Annamaria Ventura 4 , Maria Ciccarelli 4 , Laura Piacente 4 , Silvia Colucci 1 , Luciano Cavallo 4 , Maria Grano 1 & Maria Felicia Faienza 4
1Section of Human Anatomy and Histology, Department of Basic Medical Sciences, Neurosciences and Sense Organs, University ‘A. Moro’ of Bari, Bari, Italy; 2Pediatric Unit, IRCCS Casa Sollievo della Sofferenza,
San Giovanni Rotondo, Italy; 3Italian Auxological Institute Foundation, Research Institute, Verbania, Italy; 4Department of Pediatrics, Hospital Giovanni XXIII-Policlinico of Bari, University ‘A. Moro’ of Bari, Bari, Italy.
Background: Obesity and in particular visceral adiposity has been related to low bone mineral density (BMD) and greater fracture risk. Subjects with Prader–Willi syndrome (PWS) have lower amount of visceral fat than patients with simple obesity, however can develop osteoporosis. A strong relationship between inhibition of the osteoblast formation and induction of the adipocyte differentiation has been demonstrated. Inhibitors of osteoblastogenesis, such as Dickkopf-1 (DKK1), a Wnt-signaling antagonist, can increase the formation of adipocytes.
Objective and hypotheses: We aimed to analyze the serum levels as well as the expression of DKK1 in obese and in PWS subjects.
Methods: We studied by flow cytometry the expression of DKK1 in peripheral blood cells (PBCs) from 22 obese children (10 M, 9.5±3.2 years, BMI S.D. 1.9–2.6), 12 PWS adults (4 M, 29.5±6.4 years, BMI 30.8–65.7 kg/m2), 6 PWS children (2 M, 8.3±3.0 years, BMI S.D. 0.82–4.92), as well as 20 controls sex and age matched to obese and PWS children. DKK1 levels were also measured in the sera from patients and controls.
Results: Flow cytometry analysis demonstrated that monocytes, T-lymphocytes and neutrophils from PWS and obese children expressed higher levels of DKK1 respect to controls (P<0.01). Interestingly, serum DKK1 concentrations were significantly higher in obese children than in controls (P<0.01), but not in PWS patients. Obese children showed an inverse correlation between BMD and abdominal obesity, and PWS adults showed a lower BMD than PWS children.
Conclusions: Our preliminary results highlight the high expression of DKK1 in PBCs from obese and PWS patients, which may have a prominent role in the increased fat depots in both of these subjects. Higher serum levels of DKK1 in obese than PWS subjects could explain the different distribution of adiposity in simply obesity respect to PWS.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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