SFEBES2014 Poster Presentations Clinical biochemistry (21 abstracts)
Serum 5HIAA: a better biomarker than urine for detecting and monitoring neuroendocrine tumours?
Joanne Adaway 1, , Rebecca Dobson 4 , Jennifer Walsh 3 , Daniel Cuthbertson 4 , Philip Monaghan 5 , Juan Valle 2, , Neil Devlin 5 & Brian Keevil 1,
1Wythenshawe Hospital, Manchester, UK; 2Manchester Academic Health Science Centre, Manchester, UK; 3Sheffield Teaching Hospitals NHS Trust, Sheffield, UK; 4Aintree University Hospital NHS Trust, Liverpool, UK; 5Christie Hospital NHS Foundation Trust, Manchester, UK.
Background: 5-hydroxyindole acetic acid (5-HIAA) (a metabolite of serotonin) is used as a marker for patients with serotonin-secreting neuroendocrine tumours. Currently, most laboratories measure 24 h 5-HIAA excretion in urine samples. Urine collections are cumbersome for the patient and impact on their daily activities; they are consequently often poorly performed, leading to over- or under-collection of urine and inaccurate 5-HIAA excretion results. Furthermore, large volumes of urine present a health and safety challenge to processing laboratories. We compared urinary and serum 5-HIAA using previously published LC–MS/MS methods to assess whether serum 5-HIAA could replace urinary 5-HIAA estimation in the diagnosis and monitoring of neuroendocrine tumours.
Methods: We measured 5-HIAA in 177 paired serum and urine samples from healthy volunteers and patients with known 5-HIAA secreting neuroendocrine tumours.
Results: The results were expressed as a percentage of the reference range and linear regression showed a correlation coefficient of 0.67 with a Passing Bablock regression equation of Serum 5-HIAA=1.61×urine 5-HIAA-0.32. Bland Altman analysis showed a proportional positive bias in the serum 5-HIAA results, with the bias increasing with the 5-HIAA concentration.
Conclusion: We have demonstrated a strong correlation between serum and urine 5-HIAA results. In patients with 5-HIAA concentrations above the reference range, the increase in serum 5-HIAA is greater than that of urinary 5-HIAA, indicating that plasma 5-HIAA may be preferable in the diagnosis and monitoring of neuroendocrine tumours. Furthermore serum 5-HIAA measurement offers greater patient convenience, and may be a better marker than urinary 5-HIAA excretion.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more