SFEBES2014 Poster Presentations Steroids (39 abstracts)
The effect of green tea extracts on steroidogenesis, proliferation and apoptosis in ovarian granulosa cells
Attila Kadasi 1, , Nora Maruniakova 1 , Adriana Kolesarova 1 , Aneta Stochmalova 2, , Richard Alexa 2, , Roland Grossmann 3 & Alexander V Sirotkin 2,
1Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovakia; 2Department of Zoology and Anthropology, Constantine the Philosopher University, Nitra, Slovakia; 3Department of Functional Genomics and Bioregulation, Institute of Animal Science, Neustadt, Germany; 4Institute for Genetics and Reproduction of Farm Animals, Animal Production Research Centre, Luzianky near Nitra, Slovakia.
The aim of our study was to examine the influence of green tea molecules on basic ovarian functions. For this purpose we have examine the effect of green tea extract, fraction of antioxidants (GTAO) and green tea fraction of polyphenols (GTPP) (at the doses 0, 1, 10, and 100 μg/ml) on proliferation, apoptosis, and steroidogenesis by cultured porcine ovarian granulosa cells. Markers of proliferation (PCNA) and apoptosis (bax) were detected by immunocytochemistry. Secretion of steroid hormones (progesterone and testosterone) was measured by RIA.
It was observed, that both GTAO and GTPP addition diminished the percentage of proliferative (PCNA-positive) granulosa cells and increased the occurence of apoptosis (proportion of bax-positive cells).
Addition of GTAO increased progesterone release and decreased testosterone. GTPP treatment stimulated progestin but reduced androgen.
These observations suggest that green tea antioxidants and polyphenols can affect basic ovarian cell functions. They can induce the suppression of proliferation, stimulation of apoptosis, promotion of progestagen and changes in testosteron release. The suppressive influence of green tea consumption on reproductive functions are therefore not to be excluded.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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