BSPED2013 Oral Communications Oral Communications 4 (6 abstracts)
Increased urinary megalin and cubulin excretion in children with type 1 diabetes mellitus: an association with low molecular weight protein loss
Ernestas Sirka , Victoria Manwaring , Catherine Peters , Rakesh Amin , Wendy Heywood , Peter Hindmarsh & Kevin Mills
UCL Institute of Child Health, London, UK.
Nephropathy remains a major diabetes related complication despite improvements in metabolic control. Current interventions are based on the appearance of albuminuria. Whether earlier detection and treatment might be beneficial is unclear. The reabsorption of low molecular weight (<70 kDa) plasma proteins in the renal proximal tubule is mediated by an endocytic receptor, megalin, and its coreceptor, cubulin.
Using label free quantitative proteomics we measured megalin, cubulin, vitamin-D binding protein, retinal binding protein and albumin in the urine of ten children with type 1 diabetes mellitus (T1DM) and ten controls. The T1DM children displayed normal glomerular filtration rates with no evidence of microalbuminuria. Urinary megalin (T1DM 300 pmol/mmol creatinine; controls 110 pmol/mmol creatinine) and cubulin (450 vs 50 pmol/mmol creatinine; P=0.05) values were elevated compared to controls. Excretion of vitamin-D binding protein and retinal binding protein were increased in T1DM (100 and 6.2 pmol/mmol creatinine respectively) compared to controls (40 and 1.0 pmol/mmol creatinine respectively; P=0.05). Albumin excretion was not increased significantly. No correlation with HbA1c was observed.
Increased urinary excretion of low molecular weight proteins such as megalin and cubulin in children with T1DM may presage microalbuminuria.
Volume 33
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more