ECE2013 Poster Presentations Obesity (65 abstracts)
The effect of rutin on homocysteine induced proliferation of 3T3-L1 preadipocyte in vitro
Sung Hoon Yu 1 , Jun Goo Kang 1 , Yoo-Cheol Hwang 2 , Hong Yup Ahn 3 , Cheol-Young Park 4 , Dong Sun Kim 5 & Hyung Joon Yoo 1
1Division of Endocrinology and Metabolism, Department of Internal Medicine, Hallym University College of Medicine, Seoul, Republic of Korea; 2Division of Endocrinology and Metabolism, Department of Medicine, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, Seoul, Republic of Korea; 3Department of Statistics, Dongguk University-Seoul, Seoul, Republic of Korea; 4Division of Endocrinology and Metabolism, Department of Internal Medicine, School of Medicine Sungkyunkwan University, Seoul, Republic of Korea; 5Division of Endocrinology and Metabolism, Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Republic of Korea.
Introduction: Rutin (C27H30O16) is the major representative of the flavonol subclass of flavonoids. Rutin has wide variety of pharmacological activity, however there are not sufficient data in 3T3-L1 preadipocyte. In this study, we demonstrated that the effect of rutin on the proliferation of 3T3-L1 preadipocyte.
Methods: The 3T3-L1 preadipocyte were treated with homocysteine (1 mM), rutin (30 μM) and rutin after homocysteine. And we analyzed the cell proliferation with methylthiazoletetrazolium (MTT) assay. We evaluated the proliferation pathway and apoptotic pathway with Western blot.
Results: The proliferation of 3T3-L1 preadipocyte was enhanced with homocysteine (P<0.001). The cell viability of 3T3-L1 preadipocyte was not changed with rutin in diverse doses (5, 10, 30, 60 μM). But rutin after homocysteine treatment significantly suppressed the proliferation of 3T3-L1 preadipocyte compared to homocysteine treatment. In Western blot analysis, Immunoexpression of phospho-p44/42 MAPK (ERK1/2) and phospho-MEK1/2 were significantly increased by homocysteine (1 mM). However, these were suppressed with PD98059 treatment, not by wortmannin treatment.
Conclusion: These resuls suggested rutin suppressed the proliferation of 3T3-L1 preadipocyte by homocysteine through p44/42 MAPK pathway.
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Endocrine Abstracts
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