ECE2013 Poster Presentations Male reproduction (41 abstracts)
Identification of vitamin D (VDR) and retinoic X (RXR) receptor in normal and neoplastic human reproductive tissues
Federica Cariati 1 , Vincenzo Gigantino 2 , Giorgio Coppola 1 , Claudia Pivonello 1 , Mariano Galdiero 1 , Gerardo Botti 2 , Loredana Gandini 3 , Andrea Lenzi 3 , Renato Franco 2 , Annamaria Colao 1 & Rosario Pivonello 1
1Section of Endocrinology, Department of Clinical Medicine and Surgery, Federico II Universty, Naples, Italy; 2Pathology Unit, National Cancer Institute, Pascale Foundation, Naples, Italy; 3Department of Experimental Medicine, University of Rome ‘La Sapienza’, Rome, Italy.
Background: Vitamin D is an important modulator of cell growth, differentiation and death in normal and neoplastic cells. Its actions are mediated by vitamin D receptor (VDR), which heterodimerizes with nuclear retinoid X receptors (RXRα). Recently, it has been suggested that vitamin D system have a role in male reproduction. The aim of this study was to investigate the VDR and RXRα localization in normal and neoplastic human male reproductive tissues.
Methods: Identification of VDR and RXRα was performed by immunohistochemistry and immunofluorescence on epididymis, seminal vesicle, testis and prostate healthy tissues and on two tissue micro arrays (TMA) platforms. TMAs were built using the most representative areas from testicular germ cell cancer (TGCTs) samples (15 carcinomas in situ, 25 embryonal carcinomas, 39 seminomas, 5 choriocarcinomas, 15 teratomas, and 15 Yolk sac tumours) and from 40 cases of prostate cancer (PC).
Results: VDR and RXRα were expressed in epididymis, seminal vesicle and prostate tissues. VDR showed membrane-cytoplasmic immunoreactivity while RXRα showed a cytoplasmic localization. In normal testis, VDR was expressed in Sertoli cells and in epithelium of rete testis while RXRα was detected in Leydig, Sertoli and spermatogonia cells. Membrane VDR immunostaining was strong in differentiated TGCTs while RXRα localization was much more positive in undifferentiated TGCTs. Notably, VDR showed high expression in healthy prostate (80%±0) compared to PC tissues (64%±16.8). Moreover, VDR expression decreased in tumours with high Gleason score (GS) (67% in GS<7; 50% in GS=7 and 30% in GS>7) and in these latter VDR was localized exclusively on membrane.
Conclusions: VDR and RXR are expressed in different tissue of the reproductive system and tumours, suggesting a role of vitamin D in reproduction and/or in the development of tumors. Membrane VDR localization suggest a non-genomic role of VDR in prostate and testis tumour tissues.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more