Endocrine Abstracts

The oral antidiabetic drug, glibenclamide, stimulates the insulin producing β-cells constantly through a harsh mechanism which eventually may permanently reverse its endocrinofunction. The study investigates the protective effects from Swietenia seed and endocarp aqueous-methanol extract on pancreatic islets histology in rats of type 2 DM model. Phytochemical analyses were performed prior to the in vivo study to screen the aqueous-methanolic extract of the combined plant parts. The experimental groups were rendered diabetic by chemical combination of STZ (65 mg/kg bwt, i.v.) and NAD (230 mg/kg bwt, i.p.) in adult rats. Diabetic rats were orally force-fed with glibenclamide (5 mg/kg bwt) or extracts (250 mg/kg bwt) daily for 3 weeks. Body weight (g) and FBG levels (mg/dl) were determined at treatment intervals of 0, 7, 14 and 21. Subsequently, Langerhans’ islets were examined by H&E staining. Photomicrographs of pancreatic islet revealed that administration of extracts showed improved cellular density suggesting that the extracts were capable of inducing β-cells recovery and/or regeneration following destructive effects of STZ. Findings indicate that Swietenia macrophylla King seed and endocarp aqueous-methanolic extract exhibits protective effect on islet histology and were also involved in correction of altered biological parameters. Hence, it may serve as candidate for developing a safe, complaisance and promising neutraceutical for the management of diabetes.

Key Words: Swietenia macrophylla King, Diabetes, islet of Langerhans, Streptozotocin, Rats.