ECE2013 Poster Presentations Thyroid cancer (64 abstracts)
Pre-miR146a expression profiling of follicular thyroid carcinoma
Elisa Pignatti 1 , Eleonora Vighi 1 , Luca Roncati 2 , Elda Kara 1, , Eleonora Porcheddu 3 , Elisa Magnani 1 , Vincenzo Rochira 1, , Antonino Maiorana 2 , Cesare Carani 1, & Manuela Simoni 1,
1Chair and Unit of Endocrinology & Metabolism, Department of Biomedical, Metabolic and Neural Sciences, University of Modena & Reggio Emilia, Modena, Italy; 2Department of Diagnostic Medicine, Clinical and Public Health, University of Modena & Reggio Emilia, Modena, Italy; 3Department of Life Sciences, University of Modena & Reggio Emilia, Modena, Italy; 4Integrated Department of Medicine, Endocrinology and Metabolism, Geriatrics, Azienda USL of Modena, NOCSAE of Baggiovara, Modena, Italy.
Introduction: Follicular thyroid carcinomas (FTC) are the second most common type of thyroid malignancy. Micro-RNAs (miRNAs) are a new class of small, noncoding RNAs whose expression is deregulated in many types of human cancers and may lend novel clues to FTC genesis. Pre-miR146a represents one of the most up-regulated miRNAs in papillary thyroid carcinomas (PTC) and a SNP (rs2910164), identified in pre-miR146a, contributes to genetic predisposition to PTC, but data on FTC are still lacking. The objective of the study was to evaluate the expression of pre-miR146a in FTC both in neoplstic and non-neoplastic tissues that in relation to the rs2910164 SNP of pre-miR146a.
Design: Pre-miR146a expression levels were detected in 35 male and female patients with FTC, aged 53±18. RNA was extracted from surgically removed thyroid neoplastic and non-neoplastic FFPE samples. Total RNA containing miRNAs was used for stem-loop RT reactions. A standard TaqMan PCR kit protocol was implemented for real-time PCR. Reactions were performed on the CFX96 Real-Time System (Bio-Rad) with U6 RNA as an endogenous loading control. The pre-miR146a common G/C polymorphism, designated rs2910164, was genotyped by sequencing. Wilcoxon signed-rank test and Friedman test were used for statistical analysis.
Results: The expression of pre-miR146a is significantly down regulated in tumor compared to non-neoplastic tissues in patients with FTC (P=0.043). rs2910164 genotype is related neither to the level of expression of pre-miR146a nor to the type of tissue analyzed. Finally, no correlations between the expression of pre-miR146a and the genotype of the SNP in the transition from non-neoplastic to neoplastic tissue in patients with FTC were found.
Conclusions: The expression of pre-miR146a is related to FTC but not to the rs2910164 SNP.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more