ECE2013 Poster Presentations Thyroid cancer (64 abstracts)
Inhibition of proliferation in anaplastic thyroid cell lines
Joana Rodrigues 1 , Angela Rodriguez 1 , Sihara Perez 1 , Francisco Barreiro 2 , Jose Cameselle-Teijeiro 3 , Maria Rodriguez 1 , Susana Bravo 1 & Clara Alvarez 1
1Physiology, IDIS-CIMUS, School of Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain; 2Surgery, IDIS-Hospital Clinico Universitario, University of Santiago de Compostela, Santiago de Compostela, Spain; 3Pathology, IDIS-Hospital Clinico Universitario, University of Santiago de Compostela, Santiago de Compostela, Spain.
Introduction: Our group has a bank of human primary thyroid cultures (BANTTIC) obtained from patients. All are individual cultures specifically genotyped and phenotyped. We believe of great interest the search for biomarkers in these cultures using differential proteomic analysis.
We have applied differential proteomics using 2D-PAGE coupled to MALDI-TOF-TOF-MS. We have compared benign pathologies (normal thyroid and Pendred’s syndrome) and differential thyroid carcinomas (thyroid papillary carcinoma).
Individual protein quantities were evaluated by the Student’s t-test within the PDQuest analysis in order to compare the two groups and identify sets of proteins that showed a statistically significant difference with a confidence level of 0.05.
One of the differential spots we have found (spot-8002) is increased in carcinoma cultures (T-PC) vs benign pathologies.
Aim: To perform functional studies to investigate 8002 protein in anaplastic thyroid cell lines.
Results: 8002 protein is a low molecular weight variant with two isoforms. The gene encoding spot-8002 is related with nuclear functions but also it is described to bind to cytoskeleton.
Using western blot with specific antibodies for 8002 protein, experiments that compare primary thyroid cultures from BANTTIC and commercial cell lines, have showed that 8002 protein is processed and increased in Differentiated Thyroid carcinomas (DTC) and Anaplastic Thyroid Carcinoma (ATC) in comparison with Normal Thyroid (NT).
A specific 8002 siRNA was designed. When transfected in Papillary Thyroid Carcinoma, Follicular Thyroid Carcinoma and Anaplastic Thyroid Carcinoma cell lines has blocked proliferation of Anaplastic cell lines during several days.
More functional studies will be carried out to investigate this protein.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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