ECE2013 Poster Presentations Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) (17 abstracts)
8-Prenylnaringenin decreases hormone expression in GH3 pituitary adenoma cells of the rat
Rosita Rupa 1 , Benjamin Voellger 1 , Elmar Kirches 2 , Christian Mawrin 2 & Raimund Firsching 1
1Klinik fuer Neurochirurgie, Otto-von-Guericke-Universitaet Magdeburg, Magdeburg, Germany; 2Institut fuer Neuropathologie, Otto-von-Guericke-Universitaet Magdeburg, Magdeburg, Germany.
Objective: 8-Prenylnaringenin (8-PN) is a phytoestrogen discovered in hops. GH3 pituitary adenoma cells of the rat show a variety of responses to phytoestrogens in vitro. This in vitro study aims to analyze changes in hormone expression in GH3 cells treated with 8-PN.
Methods: GH3 cells were incubated with 0.5–50 μM 8-PN for 4 h. Medium and ethanol served as controls. Changes in expression of growth hormone (GH) and prolactin (PRL) normalized to β2 microglobuline (β2MG) were assessed using quantitative real time PCR (QRT-PCR). T served as statistical test. P<0.05 was supposed to be statistically significant.
Results: In GH3 cells treated with 0.5–50 μM 8-PN, expression of GH and PRL is decreased as compared to controls. Significant differences in GH expression were found after treatment with 0.5 μM 8-PN as compared to ethanol (P=0.042), 0.5 μM 8-PN as compared to medium (P=0.011), 5 μM 8-PN as compared to medium (P=0.022) and 50 μM 8-PN as compared to medium (P=0.013). Significant differences in PRL expression were found after treatment with 0.5 μM as compared to ethanol (P=0.039) and 50 μM 8-PN as compared to ethanol (P=0.046).
Conclusion: Further investigation of the impact of 8-PN on hormone expression and secretion in GH3 cells is warranted.
Volume 32
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Endocrine Abstracts
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