ECE2013 Poster Presentations Endocrine tumours and neoplasia (66 abstracts)
Pituitary transforming gene 1 (PTTG1) expression in seminoma
Domenico Milardi 1 , Giuseppe Grande 2 , Francesco Pierconti 3 , Maurizio Martini 3 , Tonia Cenci 3 , Gaetano Gulino 4 , Giovanni Schinzari 5 , Luigi Maria Larocca 3 , Guido Rindi 3 , Alfredo Pontecorvi 2 & Laura De Marinis 2
1International Scientific Institute “Paolo VI”, Catholic University of the Sacred Heart, Rome, Italy; 2Department of Clinical Medicine, Unit of Endocrinology, Catholic University of the Sacred Heart, Rome, Italy; 3Department of Pathology, Catholic University of Sacred Heart, Rome, Italy; 4Department of Urology, Catholic University of the Sacred Heart, Rome, Italy; 5Department of Internal Medicine, Unit of Medical Oncology, Catholic University of Sacred Heart, Rome, Italy.
Pituitary transforming gene 1 (PTTG1) is a mammalian securing involved in mitosis to ensure chromosomal stability. PTTG1 is overexpressed in a variety of tumors. It can directly and indirectly induce expression of genes that are involved in regulating tumorigenesis and cancer development (c-Myc, bFGF, VEGF and MMP2).
We evaluated PTTG1-expression by immunohistochemistry on formalin-fixed and paraffin-embedded specimen testicular tissues from 53 male patient underwent to therapeutic orchidectomy, collected from 2006 to 2011.
PTTG1 staining was located in the nuclear and cytoplasm of neoplastic cells. Within the tumor we identify a peripheral zone as edge of neoplasia and neoplastic tissue up to 1 mm towards interior of the tumor and central zone as neoplastic areas further than 1 mm from the border of the tumor. Different staining in subcellular localization (nuclear and cytoplasm) was observed in peripheric area vs central area of neoplastic lesion. In the peripheric area, PTTG1 immunoreactivity was detected mostly localized in the nucleus, while in the central area PTTG1 staining was evident more intensely in cytoplasm of positive elements.
PTTG1 expression was significantly lower in the central when compared with the peripheral area, with a greater number of positive cells in the borders of the tumor and gradient periphery/center significantly correlate with the size of the tumor.
PTTG1 positive staining was also reported in the peritumoral region a nuclear staining prevalent pattern.
We firstly described neoplastic PTTG-1 positive cells with nuclear and cytoplasmatic staining in seminoma. Our distribution data support the idea that PTTG1-expression in neoplastic cells in the front of the tumor infiltration and in the intertubular areas might respond to demand of the tumor cells to move and invade surrounding tissue, increasing tumor angiogenesis. Further investigation are required to clarify if a functional abrogation of PTTG-1 could provide new therapeutic approaches in the management of seminoma.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more